Culter alburnus
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ABSTRACT:
PROVIDER: PRJNA300320 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Megalobrama amblycephala x Culter alburnus Transcriptome or Gene expression
| PRJNA269572 | ENA
Project description:Culter alburnus isolate:S04 | cultivar:Erythroculter ilishaeformis Raw sequence reads
| PRJNA340306 | ENA
Project description:The changes in the expression of proteins in the eggs of C. alburnus-B and C. alburnus-A at four time points (unfertilized, and 0, 0.5 and 1 h postfertilization, n=3) were quantified by TMT-based proteomics, and the changes in the expression of proteins in the egg envelopes of C. alburnus-B and C. alburnus-A at 1 h after fertilization were quantified by label-free proteomics.
2022-10-13 | PXD023609 | Pride
Project description:Nucleolar dominance is an epigenetic phenomenon that occurs in interspecific hybrids and involves the expression of 45S rRNA genes inherited from one progenitor due to the silencing of the other progenitor's rRNA genes. In this paper, changes in the genetics and expression of 45S rRNA genes in F1 and F2 hybrid progeny of blunt snout bream (BSB, Megalobrama amblycephala)?×?topmouth culter (TC, Culter alburnus) are investigated.The 45S rDNA loci were analyzed by cloning, RT-PCR and sequencing methods. The results show that nucleolar dominance patterns differ in the F1 and F2 hybrids. In the F1 hybrids of BSB?×?TC, all the tested individuals inherited and expressed the 45S rRNA genes of both BSB and TC, indicating that nucleolar dominance is not established in the F1 hybrids. However, in the F2 hybrids of BSB?×?TC, five patterns are observed. Pattern 1 inherits and expresses only the 45S rRNA gene of BSB. Pattern 2 inherits the 45S rRNA gene from both BSB and TC, but only expresses the 45S rRNA of BSB. Pattern 3 inherits and expresses the 45S rRNA gene from both BSB and TC. Pattern 4 inherits the 45S rRNA gene from both BSB and TC, but only expresses the 45S rRNA gene of TC. Pattern 5 inherits and expresses only the 45S rRNA gene of TC.Nucleolar dominance shows distinctive patterns in intergeneric hybrids of BSB?×?TC. It is not established in F1 hybrids and is random in F2 hybrids. This study provides new insights into the phenomenon of nucleolar dominance in genetic hybrids in vertebrates.
| S-EPMC5139125 | biostudies-literature
Project description:Melanocortin-4 receptor (MC4R) plays important roles in regulation of multiple physiological processes, and interaction of MC4R and melanocortin receptor accessory protein 2 (MRAP2) is suggested to play pivotal role in energy balance of vertebrates. Topmouth culter (Culter alburnus) is an economically important freshwater fish in China. Herein we cloned culter mc4r, mrap2a, and mrap2b. Culter mc4r consisted of a 981 bp open reading frame encoding a protein of 326 amino acids. qRT-PCR revealed that mc4r, mrap2a, and mrap2b were primarily expressed in the central nervous system. In the periphery, mc4r and mrap2b were expressed more widely in the male, while mrap2a was expressed more widely in the female. Culter MC4R could bind to four peptide agonists and increase intracellular cAMP production dose dependently. Culter MC4R was constitutively active in both cAMP and ERK1/2 pathways, which was differentially regulated by culter MRAP2a and MRAP2b. Culter MRAP2a significantly increased Bmax and decreased agonist-stimulated cAMP, while MRAP2b increased cell surface and total expression but did not affect Bmax and agonist-stimulated cAMP. These results will aid the investigation of the potential physiological processes that MC4R might be involved in topmouth culter.
| S-EPMC7456811 | biostudies-literature