Project description:To study the epigenetic regulation during neuroectoderm specification process, we generated in-depth maps of nucleosome and histone modifications.We found that the depletion of nucleosome marked with H2A.Z in promoter regions contributes to the activation of key neuroectoderm related genes as providing open chromatin state for Pol II binding. Meanwhile, HMs undergo active remodeling with recruiting key transcription factors.So our study provided a full view on the transcriptional regulation of epigenome during neuroectoderm specification.
Project description:To study the epigenetic regulation during neuroectoderm specification process, we generated in-depth maps of nucleosome and histone modifications.We found that the depletion of nucleosome marked with H2A.Z in promoter regions contributes to the activation of key neuroectoderm related genes as providing open chromatin state for Pol II binding. Meanwhile, HMs undergo active remodeling with recruiting key transcription factors.So our study provided a full view on the transcriptional regulation of epigenome during neuroectoderm specification.
Project description:We generated genome-wide chromatin state and RNA Polymerase II binding maps in mouse erythroid cells by ChIP-Seq. Examination of 4 different histone modifications (H3K4me3, H3K4me1, H3K27me3, H3K27ac) and RNA Polymerase II (RNAP2) binding in mouse erythroid cells (Ter119+).
Project description:We generated genome-wide H3K9me3-state maps of DP thymocytes purified from ESET+/+ and ESET-/- mice by using next generation sequencing. Examination of H3K9 trimethylation in DP thymocytes purified from ESET+/+ and ESET-/- mice.
Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone H3 trimethylation in rice endosperm. By obtaining about four hundred million bases of sequence from rice chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of rice endosperm. We find that the presence of H3K27me3 in either upstream or downstream of a gene is predominately associated with repression of the gene, while its absence is mainly associated with high gene expression. Examination of Histone H3 lysine 27 trimethylation in rice endosperm.