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Leucaena leucocephala

ABSTRACT: Leucaena leucocephala Organism overview

PROVIDER: PRJNA308772 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2517688_1.fastq.gz	Fastqsanger.gz
	SRR2517688_2.fastq.gz	Fastqsanger.gz
	SRR2517689_1.fastq.gz	Fastqsanger.gz
	SRR2517689_2.fastq.gz	Fastqsanger.gz

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Leucaena leucocephala

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Determination of genes expressed in response to drought in root and shoot of Leucaena leucocephala

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Determination of genes expressed in response to drought in root and shoot of Leucaena leucocephala

Project description:Leucaena leucocephala seedlings were treated with PEG6000 and the shoot and root tissues were collected after 48 hours following the treatment. The gene expressions were compared between treated and untreated in root and shoot separately. The differentially expressed genes may be related to drought resistance. RNA from shoot and root from treated and untreated L. leucocephala seedlings were extracted. Two biological replicates were made for each sample (each replicate represents about 10 individual seedlings).

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Jasmonate-Elicited Stress Induces Metabolic Change in the Leaves of Leucaena leucocephala.

Project description:The plant Leucaena leucocephala was exposed to four jasmonate elicitors, i.e., jasmonic acid (JA), methyl jasmonic acid (MeJA), jasmonoyl-l-isoleucine (JA-Ile) and 6-ethyl indanoyl glycine conjugate (2-[(6-ethyl-1-oxo-indane-4-carbonyl)-amino]-acetic acid methyl ester) (CGM). The treatment was to mimic the herbivores and wounding stresses. By using NMR spectroscopy along with chemometric analysis, including principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA), the changes of metabolites in the leaves of L. leucocephala were determined under the stress as induced by the four elicitors. The challenge of JA-Ile caused an accumulation of lactic acid (6), ?-glucose (10), alanine (12), threonine (13), steroids (18), 3,4-dihydroxypyridine (19) and an unidentified compound 20. The chemometric analysis of the PCA and PLS-DA models indicated that the alternation of metabolites triggered by JA, MeJA, and CGM treatments were very minimum. In contrast, the treatment by JA-Ile could induce the most significant metabolic changes in the leaves. Moreover, there was very minimal new metabolite being detected in responding to the jasmonate-induced stresses. The results showed some metabolite concentrations changed after application of the elicitors, which may be related to a high level of tolerance to stress conditions as well as the strong ecological suitability of L. leucocephala.

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