Project description:Cryptosporidium andersoni initiates infection first caused by the release of sporozoites by excystation. However, the proteins involved in excystation remain unknown. The research of the molecules that participate in the excystation of C. andersoni oocysts will fill a gap in our understanding of the excyst system of this parasitic pathogen
Project description:Cryptosporidium is a leading cause of diarrheal disease in children and an important contributor to early childhood mortality. The parasite invades intestinal epithelial cells and remodels them extensively including building an elaborate interface structure. How this occurs at the molecular level is largely unknown. Here, we generated a whole-cell spatial proteome of the Cryptosporidium sporozoite using the spatial proteomics technology hyperLOPIT. These data in combination with genetic and cell-biological experimentation enabled the discovery of the Cryptosporidium secreted effector proteome, including a new secretory organelle.