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Paulinella chromatophora strain:CCAC0185

ABSTRACT: Paulinella chromatophora strain:CCAC0185 Transcriptome or Gene expression

PROVIDER: PRJNA311736 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3217360_1.fastq.gz	Fastqsanger.gz
	SRR3217360_2.fastq.gz	Fastqsanger.gz
	SRR3217341_1.fastq.gz	Fastqsanger.gz
	SRR3217341_2.fastq.gz	Fastqsanger.gz
	SRR3217293_1.fastq.gz	Fastqsanger.gz

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The Paulinella chromatophore N-terminome

Project description:The cercozoan amoeba Paulinella chromatophora contains photosynthetic organelles - termed chromatophores - that evolved from a cyanobacterium ~100 million years ago, independently from plastids in plants and algae. Despite its more recent origin, at least one third of the chromatophore proteome consists of nucleus-encoded proteins that are imported by an unknown mechanism across the chromatophore double envelope membranes. Chromatophore-targeted proteins fall into two classes. Proteins exceeding 250 amino acids carry a conserved N-terminal sequence extension, termed the ‘chromatophore transit peptide’ (crTP), that is presumably involved in guiding these proteins into the chromatophore. Short imported proteins do not carry discernable targeting signals. To explore whether the import of protein is accompanied by their N-terminal processing, here we used a mass spectrometry-based approach to determine protein N-termini in Paulinella chromatophora and identified N-termini of 208 chromatophore-localized proteins. Our study revealed extensive N-terminal modifications by acetylation and proteolytic processing in both, the nucleus and chromatophore-encoded fraction of the chromatophore proteome. Mature N-termini of 37 crTP-carrying proteins were identified, of which 30 were cleaved in a common processing region. Our results imply that the crTP mediates trafficking through the Golgi, is bipartite and surprisingly only the N-terminal third (‘part 1’) becomes cleaved upon import, whereas the rest (‘part 2’) remains at the mature proteins. In contrast, short imported proteins remain largely unprocessed. Finally, this work sheds light on N-terminal processing of proteins encoded in an evolutionary-early-stage photosynthetic organelle and suggests host-derived post-translationally acting factors involved in dynamic regulation of the chromatophore-encoded chromatophore proteome.

2023-04-06 | PXD028527 | Pride

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