Polypedilum vanderplanki
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PROVIDER: PRJNA313948 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Polypedilum vanderplanki transcriptome during desiccation-rehydration cycle
| PRJNA312200 | ENA
Project description:Transcriptomic analysis of anhydrobiotic gene regulatory network for cultured cell line, Pv11, from Polypedilum vanderplanki
| PRJDB8758 | ENA
Project description:Polypedilum vanderplanki is а striking and unique example of an insect that can survive almost complete water loss. Its genome and series of dehydration-rehydration transcriptomes, together with the genome of P. nubifer (congeneric desiccation-sensitive midge), were recently released. Here, using series of data reflecting detailed transcriptome changes in the process of anhydrobiosis, as well as developmental series, we have identified hundreds of new genes and have demonstrated that up to 53% of genes undergo alternative splicing (AS) and that AS plays a prominent role in the desiccation response. We have shown that the TCTAGAA DNA motif, which closely resembles the binding motif of the D. melanogaster heat shock transcription activator (HSTF), is significantly enriched in promoter regions of desiccation-induced genes, such as LEA, thioredoxins or trehalose metabolism-related genes in P. vanderplanki, but not in P. nubifer. Unlike desiccation-senseitive P. nubifer, P. vanderplanki exhibits double TCTAGAA sites upstream of the HSTF gene, that is a likely explanation for the much stronger activation of HSTF in P. vanderplanki compared to P. nubifer under desiccation. Thus, our results show that rewiring of the heat shock regulatory system is an important evolutionary mechanism of desiccation adaptation in P. vanderplanki.
2018-06-30 | GSE78799 | GEO
Project description:Genome analysis of desiccation tolerant midge, Polypedilum vanderplanki
| PRJDB1558 | ENA
Project description:Transcriptomic analysis of anhydrobiosis for cultured cell line, Pv11, from Polypedilum vanderplanki
| PRJDB7516 | ENA
Project description:RNA-Seq of Pv11 cells derived from the anhydrobiotic organism Polypedilum vanderplank, exposed to various stresses.
2022-03-07 | GSE158443 | GEO
Project description:Voit2003 - Trehalose Cycle
This model is described in the article:
Biochemical and genomic
regulation of the trehalose cycle in yeast: review of
observations and canonical model analysis.
Voit EO.
J. Theor. Biol. 2003 Jul; 223(1):
55-78
Abstract:
The physiological hallmark of heat-shock response in yeast
is a rapid, enormous increase in the concentration of
trehalose. Normally found in growing yeast cells and other
organisms only as traces, trehalose becomes a crucial protector
of proteins and membranes against a variety of stresses,
including heat, cold, starvation, desiccation, osmotic or
oxidative stress, and exposure to toxicants. Trehalose is
produced from glucose 6-phosphate and uridine diphosphate
glucose in a two-step process, and recycled to glucose by
trehalases. Even though the trehalose cycle consists of only a
few metabolites and enzymatic steps, its regulatory structure
and operation are surprisingly complex. The article begins with
a review of experimental observations on the regulation of the
trehalose cycle in yeast and proposes a canonical model for its
analysis. The first part of this analysis demonstrates the
benefits of the various regulatory features by means of
controlled comparisons with models of otherwise equivalent
pathways lacking these features. The second part elucidates the
significance of the expression pattern of the trehalose cycle
genes in response to heat shock. Interestingly, the genes
contributing to trehalose formation are up-regulated to very
different degrees, and even the trehalose degrading trehalases
show drastically increased activity during heat-shock response.
Again using the method of controlled comparisons, the model
provides rationale for the observed pattern of gene expression
and reveals benefits of the counterintuitive trehalase
up-regulation.
To
induce a heat shock, set the parameter heat_shock from 0 to 1. This
changes the parameter values of X8 to X19 from 1 to the values
given in table 3 of the original publication.
As
this is an S-systems model, it does not contain any reactions
encoded in SBML.
This model is hosted on
BioModels Database
and identified by:
BIOMD0000000266.
To cite BioModels Database, please use:
BioModels Database:
An enhanced, curated and annotated resource for published
quantitative kinetic models.
To the extent possible under law, all copyright and related or
neighbouring rights to this encoded model have been dedicated to
the public domain worldwide. Please refer to
CC0
Public Domain Dedication for more information.
2024-09-02 | BIOMD0000000266 | BioModels
Project description:Polypedilum vanderplanki is a striking and unique example of an insect that can survive almost complete desiccation. Its genome and a set of dehydration-rehydration transcriptomes, together with the genome of Polypedilum nubifer (a congeneric desiccation-sensitive midge), were recently released. Here, using published and newly generated datasets reflecting detailed transcriptome changes during anhydrobiosis, as well as a developmental series, we show that the TCTAGAA DNA motif, which closely resembles the binding motif of the Drosophila melanogaster heat shock transcription activator (Hsf), is significantly enriched in the promoter regions of desiccation-induced genes in P. vanderplanki, such as genes encoding late embryogenesis abundant (LEA) proteins, thioredoxins, or trehalose metabolism-related genes, but not in P. nubifer Unlike P. nubifer, P. vanderplanki has double TCTAGAA sites upstream of the Hsf gene itself, which is probably responsible for the stronger activation of Hsf in P. vanderplanki during desiccation compared with P. nubifer To confirm the role of Hsf in desiccation-induced gene activation, we used the Pv11 cell line, derived from P. vanderplanki embryo. After preincubation with trehalose, Pv11 cells can enter anhydrobiosis and survive desiccation. We showed that Hsf knockdown suppresses trehalose-induced activation of multiple predicted Hsf targets (including P. vanderplanki-specific LEA protein genes) and reduces the desiccation survival rate of Pv11 cells fivefold. Thus, cooption of the heat shock regulatory system has been an important evolutionary mechanism for adaptation to desiccation in P. vanderplanki.
| S-EPMC5877948 | biostudies-literature