Bison bonasus
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ABSTRACT:
PROVIDER: PRJNA314040 | ENA |
REPOSITORIES: ENA
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Project description:The study was performed on a male European bison (Bison bonasus bonasus L.) foetus spontaneously aborted at the fourth or fifth month of pregnancy in the Bia?owie?a Forest. Serum samples from the foetus and mother revealed the presence of antibodies against T. gondii (S/P% = 88% and 75%, respectively). Mobile extracellular tachyzoites were first observed in a Vero cell culture, 110 days following inoculation of brain homogenate. PCR amplification with TGR1E1 and TGR1E2 primers confirmed the presence of T. gondii DNA, which was classified as Type I by PCR-RFLP genotyping. The sequences of 18S ribosomal RNA (18S rRNA) and 5.8S ribosomal RNA (5.8S rRNA) genes; internal transcribed spacer 1 (ITS1) and internal transcribed spacer 2 (ITS2), obtained from T. gondii isolate, have been deposited in GenBank (accession number KX459518.1). This is the first in vitro isolation and molecular identification of T. gondii from an aborted European bison foetus. The origin of this protozoan isolate indicates that the species is a significant threat to the European bison conservation program implemented in the Bia?owie?a Forest.
| S-EPMC5548833 | biostudies-literature
Project description:Daft genome of European bison (wisent), Bison bonasus
| PRJNA327000 | ENA
Project description:BackgroundOur recent macro- and scanning electron microscopic study of tongue conducted on domesticated cattle, wild living European bison, and Bison bonasus hybrid revealed various spatial arrangement and number of gustatory and mechanical papillae between parental species and their hybrid. Furthermore, scanning electron microscopy analysis of gustatory papillae indicated the variable distribution of fungiform papillae (Fu) over the surface of the tongue, which could be significant in differentiated taste perception during feeding in studied wild living and domesticated husbandry ruminants. To specify the detailed microstructure of Fu papillae with connective tissue cores (CTC) and intraepithelial taste buds system, the first time the three-dimensional computer-aided analysis of serial histoslides resulted in the rendering of 3D reconstructions of Fu papillae.ResultsThe comparative analysis of 3D models Fu papillae conducted in six areas of lingual mucosa of each tongue revealed information about, microstructural diversity of Fu papillae in studied ruminants. The estimation of number and density of Fu papillae on tongues, rate of protrusion of papillae over mucosa, and a number of taste buds per papilla allowed to state the ventral surface of the lingual apex and posterolateral surfaces of the lingual torus as regions important in taste perception, as in the preselection of taken food, as well in the analysis of food during rumination, respectively. On the 3D models were observed three structural types of CTC of different distribution on the tongue in studied species. The quantitative data of the number of taste buds on Fu papillae have regional functional differences in the taste system important in feeding and veterinary practice. Moreover, our analysis determined specific features in examined hybrid and showed similarities of some studied features with cattle, i.e., maternal species.ConclusionsThe 3D reconstruction method used for the first time in the field of study of the lingual papillae and taste buds system can be considered as an innovative and effective tool in assessing of the microstructures of Fu papillae, and it could be suitable for further studies of taste system structures in normal and pathological condition.
| S-EPMC8740503 | biostudies-literature
Project description:The ability to solve novel problems is crucial for individual fitness. However, studies on problem solving are usually done on few taxa, with species with low encephalization quotient being rarely tested. Here, we aimed to study problem solving in a non-domesticated ungulate species, European bison, with two experimental tasks. In the first task, five individuals were presented with a hanging barrel filled with food, which could either be directly accessed (control condition) or which could only be reached by pushing a tree stump in the enclosure below it and stepping on it (experimental condition). In the second task, five individuals were repeatedly fed by an experimenter using a novel bucket to retrieve food from a bag. Then, three identical buckets were placed in the enclosure, while the experimenter waited outside with the bag without feeding the bison, either with a bucket (control condition) or without it (experimental condition). In the first task, no bison moved the stump behind the barrel and/or stepped on it to reach the food. In the second task, two individuals solved the task by pushing the bucket within the experimenter's reach, twice in the experimental and twice in the control condition. We suggest that bison showed a limited ability to solve novel problems, and discuss the implications for their understanding of the functional aspects of the tasks.
| S-EPMC8080012 | biostudies-literature
Project description:The wisent, also known as the European bison, was rescued from extinction approximately 80 years ago through the conservation of 12 individuals. Here, we present the draft genome sequence of a male wisent individual descended from this founding stock. A total of 366 billion base pairs (Gb) of raw reads from whole-genome sequencing of this wisent were generated using the Illumina HiSeq2000 platform. The final genome assembly (2.58 Gb) is composed of 29,074 scaffolds with an N50 of 4.7 Mb. 47.3% of the genome is composed of repetitive elements. We identified 21,542 genes and 58,385 non-coding RNAs. A phylogenetic tree based on nuclear genomes indicated sister relationships between bison and wisent and between the wisent-bison clade and yak. For 75 genes we obtained evidence of positive evolution in the wisent lineage. We provide the first genome sequence and gene annotation for the wisent. The availability of these resources will be of value for the future conservation of this endangered large mammal and for reconstructing the evolutionary history of the Bovini tribe.
| S-EPMC5530314 | biostudies-literature
Project description:A newly-discovered foot disease of unknown origin in captive European Bison (Bison bonasus) was recently detected at Berne Animal Park. Dermatitis of the interdigital cleft of varying degrees of severity was diagnosed in all animals (n = 10). The aim of this study was to describe the gross and histological lesions of the interdigital cleft found in 10 captive European bison and to identify involved potential pathogens in affected feet using molecular-based methods for Treponema spp., Dichelobacter nodosus and Fusobacterium necrophorum. Lesions were scored according to the degree of gross pathology at limb level. In a single animal, the gross lesions were restricted to focal lesions on the dorsal aspect of the digital skin of each foot (score 1), whereas all other animals showed at least one foot with extended lesions including the interdigital cleft (score 2). The presence of viable spirochaetes was observed in all animals using dark field microscopy. Applying fluorescence in situ hybridisation (FISH) on biopsies, Treponema spp. were identified, infiltrating the skin lesions in varying numbers in nine animals. Nested PCRs for Treponema medium, Treponema phagedenis and Treponema pedis of swab samples showed three positive animals out of ten for the latter two, whereas pooled biopsy samples were positive in all ten animals for at least T. phagedenis (9/10) and/or T. pedis (7/10), while all samples were negative for T. medium. However, none of these Treponema species could be isolated and sequence analysis of the amplified products showed 100% match of 365 base pairs (bp) to Treponema phylotype PT3 and almost full match (530 of 532 bp, 99.6%) to Treponema phylotype PT13. The presence of T. phagedenis, PT3 and PT13 phylotypes was confirmed by FISH analyses. The phylotypes of T. phagedenis were present in all hybridized positive biopsies of Treponema spp., and PT13 and PT3 were less abundant. Neither D. nodosus nor F. necrophorum were detected. The histological Treponema score was mostly mild. Digital dermatitis in captive European Bison is contagious and differs from bovine digital dermatitis, concerning associated pathogens as well as gross appearance.
| S-EPMC8352065 | biostudies-literature
Project description:Resettlement projects of the strongly threatened European bison (Bison bonasus) require a monitoring phase to assess both population status and habitat quality. Schemes of animal body condition scores (BCS) are robust tools to meet this requirement in practice. However, so far, no BCS scheme has been designed for European bison. Here, we suggest a body condition score scheme based on the extent of soft tissue around bony structures. The scoring system was developed with scores ranging from 1 (emaciated) to 5 (obese). Condition scores can be deduced after visually assessing the European bison both from the side and behind. Robustness of the scheme was evaluated: Unbiased people from different professional backgrounds were asked to assess the BCS of photographed semiwild European bison under field conditions and results were compared. Results demonstrate the suitability of the method. Nevertheless, variability of the results among assessors illustrates the necessity for training as well as for further research to validate the scheme as a true measure of physiological condition. We discuss the prospects and limits of a broad use of this scheme within the European bison community, and recommend the BCS scheme as a management tool.
| S-EPMC6210254 | biostudies-literature