Litsea cubeba
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ABSTRACT:
PROVIDER: PRJNA319292 | ENA |
REPOSITORIES: ENA
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Project description:Litsea cubeba isolate:Lcu1 Genome sequencing and assembly
| PRJNA562049 | ENA
Project description:Litsea cubeba (Lour.) Pers. (mountain pepper, Lauraceae) is an important woody essential oil crop that produces fragrant oils in its fruits, especially in its peels. Identification of genes involved in the regulation of fruits and peel architecture is of economic significance for L. cubeba industry. It has been well known that the MADS-box genes are essential transcription factors that control flowers and fruits development. Here, we obtained 33 MADS-box genes first from the RNA-seq data in L. cubeba, and 27 of these genes were of the MIKC-type. LcMADS20, an AGAMOUS-like gene, was highly expressed in the developing stages of fruits, particularly at 85 days after full bloom. The ectopic expression of LcMADS20 in Arabidopsis resulted in not only curved leaves, early flowering and early full-opened inflorescences, but also shorter siliques and decreased percentage of peel thickness. Moreover, in the LcMADS20 transgenic Arabidopsis, the expression modes of several intrinsic ABC model class genes were influenced, among which the expression of FUL was significantly reduced and AP3, AG, and STK were significantly increased. This study systematically analyzed the MADS-box genes in L. cubeba at the transcriptional level and showed that LcMADS20 plays important roles in the regulation of fruit architecture.
| S-EPMC6893193 | biostudies-literature
Project description:The first complete chloroplast genome (cpDNA) sequence of Litsea cubeba was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 152,725 bp in length, contains a large single-copy region (LSC) of 93,673 bp, and a small single-copy region (SSC) of 18,924 bp, which were separated by a pair of inverted repeats (IR) regions of 20,064 bp, each. The genome contains 126 genes, including 82 protein-coding genes, 8 ribosomal RNA genes, and 36 transfer RNA genes. The further phylogenomic analysis showed that L. cubeba and Litsea garrettii clustered in a clade in Lauraceae family.
| S-EPMC7510600 | biostudies-literature
Project description:Litsea cubeba, an important medicinal plant, is widely used as a traditional Chinese medicine and spice. Using cytotoxicity-guided fractionation, nine new lignans 1?9 and ten known analogues 10?19 were obtained from the EtOH extract of the twigs of L. cubeba. Their structures were assigned by extensive 1D- and 2D-NMR experiments, and the absolute configurations were resolved by specific rotation and a combination of experimental and theoretically calculated electronic circular dichroism (ECD) spectra. In the cytotoxicity assay, 7',9-epoxylignans with feruloyl or cinnamoyl groups (compounds 7?9, 13 and 14) were selectively cytotoxic against NCI-H1650 cell line, while the dibenzylbutyrolactone lignans 17?19 exerted cytotoxicities against HCT-116 and A2780 cell lines. The results highlighted the structure-activity relationship importance of a feruloyl or a cinnamoyl moiety at C-9' or/and C-7 ketone in 7',9-epoxylignans. Furthermore, compound 11 was moderate active toward protein tyrosine phosphatase 1B (PTP1B) with an IC50 value of 13.5 ?M, and compounds 4?6, 11 and 12 displayed inhibitory activity against LPS-induced NO production in RAW264.7 macrophages, with IC50 values of 46.8, 50.1, 58.6, 47.5, and 66.5 ?M, respectively.
| S-EPMC6359749 | biostudies-literature