Project description:Puf3 is a RNA-binding protein, a member of the conserved Puf-protein family. Combining different functional genomics data, we have analyzed the role of Puf3 in post-transcriptional gene regulation in S. pombe. We present data on Puf3 interacting proteins and regulatory mRNA targets.
Project description:rae1 is an essential gene and encodes one of nuclear pore complex. rae1-167 mutant cells show rapid accumulation of polyA-RNA in the nucleus at 36°C followed by protein accumulation, suggesting that accumulated nuclear mRNA influences nucelar cytooplasmic transport. We used microarrays to compare the overall gene expression of rae1-167 cells grown at 36°C with that at 25°C.