Project description:To identify if genes is regulated by time of day in human tendon, RNAseq analysis was performed on biopsies taken from patella tendon 12 hours apart in young healthy subjects (9 AM and 9 PM).
Project description:To identify if genes is regulated by time of day in human tendinopathic tendon, RNAseq analysis was performed on biopsies taken from tendinopathic and contralateral patella tendon 12 hours apart in young tendinopathic subjects (9 AM and 9 PM).
Project description:To determine the LncRNA expression profile in dorsal root ganglia tissues of 7 days after rats received 150 μl of CFA (1 mg/ml Mycobacterium tuberculosis, Sigma, USA) through the patella tendon into the right knee joint. CFA injection rats macthed saline injection rats, we uesed LncRNA microArray analysis form Arraystar to examine the expression of LncRNAs and mRNAs in dorsal root ganglia tissues of 7 days after rats received CFA through the patella tendon into the right knee joint and matched Saline through the patella tendon into the right knee joint.
Project description:We wanted to infer the contribution of host vs symbiotic microbiota plasticities on thermal acclimation of the holobiont. We long-term acclimated anymals of the same clonal line to 3 different temperatures (15, 20 and 25°C) and monitored along time the changes in fitness, microbiota composition, and host transcriptome.
Project description:To determine the optimal RNA-Seq approach for animal host-bacterial symbiont analysis, we compared transcriptome bias, depth and coverage achieved by two different mRNA capture and sequencing strategies applied to the marine demosponge Amphimedon queenslandica holobiont, for which genomes of the animal host and three most abundant bacterial symbionts are available.
