Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Vibrio splendidus

ABSTRACT: Fish and shellfish pathogen

PROVIDER: PRJNA33147 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	AAMR01.dat.gz	Other
	AAMR01.fasta.gz	Fasta.gz
	AAMR01.master.dat	Other
	AFWG01.dat.gz	Other

Items per page:

1 - 5 of 224

Similar Datasets

Vibrio splendidus 12B01

Project description:Marine organism that causes disease in fish and shellfish

| PRJNA13568 | ENA

Vibrio anguillarum

Project description:Fish pathogen

| PRJNA51903 | ENA

Vibrio atlanticus LGP32

Project description:Marine organism that causes disease in fish and shellfish

| PRJEA32815 | ENA

Alginate lyases from alginate-degrading Vibrio splendidus 12B01 are endolytic.

Project description:Alginate lyases are enzymes that degrade alginate through β-elimination of the glycosidic bond into smaller oligomers. We investigated the alginate lyases from Vibrio splendidus 12B01, a marine bacterioplankton species that can grow on alginate as its sole carbon source. We identified, purified, and characterized four polysaccharide lyase family 7 alginates lyases, AlyA, AlyB, AlyD, and AlyE, from V. splendidus 12B01. The four lyases were found to have optimal activity between pH 7.5 and 8.5 and at 20 to 25°C, consistent with their use in a marine environment. AlyA, AlyB, AlyD, and AlyE were found to exhibit a turnover number (kcat) for alginate of 0.60 ± 0.02 s(-1), 3.7 ± 0.3 s(-1), 4.5 ± 0.5 s(-1), and 7.1 ± 0.2 s(-1), respectively. The Km values of AlyA, AlyB, AlyD, and AlyE toward alginate were 36 ± 7 μM, 22 ± 5 μM, 60 ± 2 μM, and 123 ± 6 μM, respectively. AlyA and AlyB were found principally to cleave the β-1,4 bonds between β-d-mannuronate and α-l-guluronate and subunits; AlyD and AlyE were found to principally cleave the α-1,4 bonds involving α-l-guluronate subunits. The four alginate lyases degrade alginate into longer chains of oligomers.

| S-EPMC4325137 | biostudies-literature

Vibrio splendidus

Project description:Vibrio splendidus polymorphism discovery sequencing

| PRJNA38539 | ENA

Vibrio splendidus

Project description:Vibrio splendidus Transcriptome or Gene expression

| PRJNA233572 | ENA

Vibrio splendidus

Project description:Splendidus pangenome

| PRJDB12639 | ENA

Vibrio splendidus

Project description:Vibrio splendidus Genome sequencing

| PRJNA481137 | ENA

Vibrio splendidus

Project description:Vibrio splendidus Genome sequencing and assembly

| PRJNA345286 | ENA

Dihydrolipoamide dehydrogenase of Vibrio splendidus is involved in adhesion to Apostichopus japonicus.

Project description:Vibrio splendidus is one of the most opportunistic marine pathogens and infects many important marine animals, including the sea cucumber Apostichopus japonicus. In this study, two genes named DLD1 and DLD2, encoding dihydrolipoamide dehydrogenase (DLD) homologues in pathogenic V. splendidus, were cloned, and conditionally expressed in Escherichia coli BL21 (DE3). The enzymatic activities of DLD1 and DLD2 showed that they both belonged to the NADH oxidase family. Both DLD1 and DLD2 were located on the outer membrane of V. splendidus as detected by whole-cell ELISA. To study the adhesion function of DLD1 and DLD2, polyclonal antibodies were prepared, and antibody block assay was performed to detect the normal function of the two proteins. DLD1 and DLD2 were determined to play important roles in adhesion to different matrices and the adhesive ability of V. splendidus reduced more than 50% when DLD1 or DLD2 was defective.

| S-EPMC6816312 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data