Activated sludge metagenome
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ABSTRACT:
PROVIDER: PRJNA340530 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Manufactured nanomaterials (MNMs) are increasingly incorporated into consumer products that are disposed into sewage. In wastewater treatment, MNMs adsorb to activated sludge biomass where they may impact biological wastewater treatment performance, including nutrient removal. Here, we studied MNM effects on bacterial polyhydroxyalkanoate (PHA), specifically polyhydroxybutyrate (PHB), biosynthesis because of its importance to enhanced biological phosphorus (P) removal (EBPR). Activated sludge was sampled from an anoxic selector of a municipal wastewater treatment plant (WWTP), and PHB-containing bacteria were concentrated by density gradient centrifugation. After starvation to decrease intracellular PHB stores, bacteria were nutritionally augmented to promote PHB biosynthesis while being exposed to either MNMs (TiO2 or Ag) or to Ag salts (each at a concentration of 5 mg L-1). Cellular PHB concentration and PhyloChip community composition were analyzed. The final bacterial community composition differed from activated sludge, demonstrating that laboratory enrichment was selective. Still, PHB was synthesized to near-activated sludge levels. Ag salts altered final bacterial communities, although MNMs did not. PHB biosynthesis was diminished with Ag (salt or MNMs), indicating the potential for Ag-MNMs to physiologically impact EBPR through the effects of dissolved Ag ions on PHB producers. 18 samples; Triplicate PHB-enriched bacterial communities recovered from activated sludge were exposed to nanoparticle (TiO2 or Ag) or AgNO3 (as a silver control) or were not exposed to an nanoparticles (control) to determine if the naoparticles affected PHB production.
2014-12-03 | E-GEOD-63781 | biostudies-arrayexpress
Project description:Active sludge microbial communities from wastewater treatment plant in Klosterneuburg, Austria - C06_H0v3 metagenome
| PRJNA340529 | ENA
Project description:Active sludge microbial communities from wastewater treatment plant in Klosterneuburg, Austria - C40_LAv3 metagenome
| PRJNA340532 | ENA
Project description:Active sludge microbial communities from wastewater treatment plant in Klosterneuburg, Austria - D04_HANv3 metagenome
| PRJNA340533 | ENA
Project description:Active sludge microbial communities from wastewater treatment plant in Klosterneuburg, Austria - C35_LANv3 metagenome
| PRJNA340531 | ENA
Project description:Manufactured nanomaterials (MNMs) are increasingly incorporated into consumer products that are disposed into sewage. In wastewater treatment, MNMs adsorb to activated sludge biomass where they may impact biological wastewater treatment performance, including nutrient removal. Here, we studied MNM effects on bacterial polyhydroxyalkanoate (PHA), specifically polyhydroxybutyrate (PHB), biosynthesis because of its importance to enhanced biological phosphorus (P) removal (EBPR). Activated sludge was sampled from an anoxic selector of a municipal wastewater treatment plant (WWTP), and PHB-containing bacteria were concentrated by density gradient centrifugation. After starvation to decrease intracellular PHB stores, bacteria were nutritionally augmented to promote PHB biosynthesis while being exposed to either MNMs (TiO2 or Ag) or to Ag salts (each at a concentration of 5 mg L-1). Cellular PHB concentration and PhyloChip community composition were analyzed. The final bacterial community composition differed from activated sludge, demonstrating that laboratory enrichment was selective. Still, PHB was synthesized to near-activated sludge levels. Ag salts altered final bacterial communities, although MNMs did not. PHB biosynthesis was diminished with Ag (salt or MNMs), indicating the potential for Ag-MNMs to physiologically impact EBPR through the effects of dissolved Ag ions on PHB producers.
2014-12-03 | GSE63781 | GEO
Project description:Active sludge microbial communities from wastewater in Klosterneuburg, Austria - Klosterneuburg WWTP active sludge D04_HANv2 metagenome
| PRJNA464892 | ENA
Project description:Active sludge microbial communities from wastewater in Klosterneuburg, Austria - Klosterneuburg WWTP active sludge C40_LAv2 metagenome
| PRJNA464896 | ENA
Project description:Active sludge microbial communities from wastewater in Klosterneuburg, Austria - Klosterneuburg WWTP active sludge D14_H0v2 metagenome
| PRJNA464897 | ENA
Project description:Active sludge microbial communities from wastewater in Klosterneuburg, Austria - Klosterneuburg WWTP active sludge D100_L0v2 metagenome
| PRJNA464895 | ENA