Project description:In the present study we used OTA producing Penicillium verrucosum to identify and quantify proteins of an organism with yet no protein information available. We were able to identify 3632 proteins in an "ab initio" translated database from DNA sequences of P. verrucosum. Additionally a SWATH analysis was done to find differentially regulated proteins at two different time points of the growth curve. We compared the proteins at the beginning and the end of the log phase.
Project description:Penicillium digitatum is the pathogen of Green mold in Postharvest citrus. After inoculating Penicillium digitatum into the wound of citrus to infect it, transcriptome sequencing was carried out and compared with the results of transcriptome sequencing of Penicillium digitatum before inoculation in order to screen the differentially expressed genes and reveal its infection mechanism.