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Vitis vinifera

ABSTRACT: O antigen acts as a shield to delay early plant immune recognition of the pathogenic bacterium, Xylella fastidiosa

PROVIDER: PRJNA345471 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4345350.fastq.gz	Fastqsanger.gz
	SRR4345360.fastq.gz	Fastqsanger.gz
	SRR4345370.fastq.gz	Fastqsanger.gz
	SRR4345380.fastq.gz	Fastqsanger.gz
	SRR4345390.fastq.gz	Fastqsanger.gz

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Similar Datasets

E-GEOD-87643

Project description:O antigen acts as a shield to delay early plant immune recognition of the pathogenic bacterium, Xylella fastidiosa

2020-04-06 | E-GEOD-87643 | ExpressionAtlas

O antigen acts as a shield to delay early plant immune recognition of the pathogenic bacterium, Xylella fastidiosa

Project description:PAMP-triggered immunity (PTI) is the first line of plant defense against invading organisms. Initiated through the perception of conserved pathogen-associated molecular patterns (PAMPs), such as lipopolysaccharide or flagellin, PTI can provide early protection against a broad range of pathogens. Active suppression of PTI by microbial effector proteins, particularly those secreted by the Type III secretion system (T3SS), is a well-known strategy employed by bacterial plant pathogens that enables them to subvert PTI and successfully colonize their hosts. In this study, we demonstrate that the xylem-limited bacterium, Xylella fastidiosa, which lacks a T3SS, utilizes an alternative strategy to delay elicitation of innate immune responses. By decorating its LPS PAMP molecule with a high molecular weight O antigen, this bacterium physically masks itself from early recognition by the grapevine innate immune system. We have elucidated the chemical structure of the O antigen and found that it is primarily an α1,2-linked rhamnan polymer. X. fastidiosa cells lacking O antigen elicited hallmarks of PTI such as ROS production, specifically in the plant xylem tissue compartment, which is comprised primarily of non-living cells. By coupling histological and genome-wide transcriptional profiling, we demonstrate that X. fastidiosa lacking its O antigen shield activates defense-related genes in grapevine. This includes a stronger and more prolonged oxidative burst at concentrations high enough to inhibit pathogen proliferation. To begin exploring translational applications of our findings, we also demonstrate that purified X. fastidiosa LPS elicitor can prime grapevine defenses, thereby conferring host tolerance to subsequent challenge with X. fastidiosa.

2018-02-22 | GSE87643 | GEO

Transcriptional responses to water deficit and Xylella fastidiosa (Pierce's disease) in Vitis vinifera

Project description:Pierce's disease, caused by the bacterium Xylella fastidiosa, is one of the most devastating diseases of cultivated grapes. To test the long-standing hypothesis that Pierce's disease results from pathogen-induced drought stress, we used the Affymetrix Vitis GeneChip to compare the transcriptional response of Vitis vinifera to Xylella infection, water deficit, or a combination of the two stresses. The results reveal a massive redirection of gene transcription involving 822 genes with a minimum 2-fold change (p<0.05), including the upregulation of transcripts for phenylpropanoid and flavonoid biosynthesis, pathogenesis related (PR) proteins, absisic acid (ABA)/jasmonic acid (JA)-responsive transcripts, and down-regulation of transcripts related to photosynthesis, growth and nutrition. Although the transcriptional response of plants to Xylella infection was largely distinct from the response of healthy plants to water stress, we find that 138 of the pathogen-induced genes exhibited a significantly stronger transcriptional response when plants were simultaneously exposed to infection and drought stress, suggesting a strong interaction between disease and water deficit. This interaction between drought stress and disease was mirrored in planta at the physiological level for aspects of water relations and photosynthesis, and in terms of the severity of disease symptoms and the extent of pathogen colonization, providing a molecular correlation of the classical concept of the disease triangle where environment impacts disease severity. Mature leaves were sampled from 2-year old V. vinifera cv. Cabernet sauvignon clone 8 vines 4 and 8 weeks post-mock or inoculation with Xylella fastidiosa (Pierce's disease). Vines were grown in growth chambers under non-water limiting and water limiting conditions (moderate and severe water stress)

2013-02-12 | E-GEOD-44213 | biostudies-arrayexpress

Vitis vinifera

Project description:Vitis vinifera transcriptome in response to Xylella fastidiosa

| PRJNA390670 | ENA

Transcriptional responses to water deficit and Xylella fastidiosa (Pierce's disease) in Vitis vinifera

Project description:Pierce’s disease, caused by the bacterium Xylella fastidiosa, is one of the most devastating diseases of cultivated grapes. To test the long-standing hypothesis that Pierce’s disease results from pathogen-induced drought stress, we used the Affymetrix Vitis GeneChip to compare the transcriptional response of Vitis vinifera to Xylella infection, water deficit, or a combination of the two stresses. The results reveal a massive redirection of gene transcription involving 822 genes with a minimum 2-fold change (p<0.05), including the upregulation of transcripts for phenylpropanoid and flavonoid biosynthesis, pathogenesis related (PR) proteins, absisic acid (ABA)/jasmonic acid (JA)-responsive transcripts, and down-regulation of transcripts related to photosynthesis, growth and nutrition. Although the transcriptional response of plants to Xylella infection was largely distinct from the response of healthy plants to water stress, we find that 138 of the pathogen-induced genes exhibited a significantly stronger transcriptional response when plants were simultaneously exposed to infection and drought stress, suggesting a strong interaction between disease and water deficit. This interaction between drought stress and disease was mirrored in planta at the physiological level for aspects of water relations and photosynthesis, and in terms of the severity of disease symptoms and the extent of pathogen colonization, providing a molecular correlation of the classical concept of the disease triangle where environment impacts disease severity.

2013-02-12 | GSE44213 | GEO

Comparative genomic hybridization of Xylella fastidiosa 9a5c (pathogenic, reference strain) and J1a12 (non-pathogenic)

Project description:Comparison of genomic DNA from two strains of Xylella fastidiosa: 9a5c (pathogenic, reference strain) and J1a12 (non-pathogenic).

2004-04-01 | E-MEXP-22 | biostudies-arrayexpress

Vitis vinifera

Project description:Transcriptional responses to water deficit and Xylella fastidiosa (Pierce's disease) in Vitis vinifera

| PRJNA189185 | ENA

Using next generation sequencing to compare gene expression of wild type and Δcsp1 mutant of bacterial plant pathogen Xylella fastidiosa strain Stag's Leap

Project description:To investigate the role(s) of a cold shock protein homolog (Csp1) in plant pathogenic bacteria Xylella fastidiosa, we compared transcriptome profiles between wild type and a csp1 deletion mutant (Δcsp1) using long read Nanopore RNA sequencing.

2021-10-10 | GSE184594 | GEO

Vitis vinifera

Project description:microRNAs associated with phosphate starvation and secondary metabolism are down-regulated in Xylella fastidiosa-infected grapevine

| PRJNA528144 | ENA

New insights on resistance mechanisms to antimicrobial compounds in a plant pathogen biofilm

Project description:Investigation of whole genome gene expression level changes in Xylella fastidiosa 9a5c biofilm, submitted to treatments with sub inhibitory and inhibitory concentrations of copper and tetracycline. A study of Xylella fastidiosa 9a5c was done using total RNA recovered from biofilm bacterial cells submitted to 3 or 7mM of CuSO4 or 100 or 800 µg/ml of tetracycline. Each chip measures the expression level of 2832 genes from Xylella fastidiosa 9a5c with thirteen 60-mer probe pairs (PM/MM) per gene, with five-fold technical redundancy.

2010-08-31 | E-GEOD-21645 | biostudies-arrayexpress

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