Project description:RNA-seq from Neurospora crassa at 5 time points of light induction, with 2 replicates for each, totalling 10 samples RNA-seq from Neurospora crassa at 5 time points of light induction, with 2 replicates for each, totalling 10 samples

2014-08-01 | E-GEOD-53534 | biostudies-arrayexpress

Characterization of Neurospora crassa ghh (NCU06095) mutant strains

Project description:To determine the genes directly and indirectly under the control of the Grainy-head homolog (GHH) transcription factor in Neurospora crassa Three different sample types (Aerial Hyphae & Conidia; Mycelia; or Whole Colonies) of both wild-type (FGSC #2489) and grainy-head homolog (FGSC #13563) strains of Neurospora crassa were subjected to transcriptome analyses to determine the genes differentially expressed in the ghh background compared to wild type.

2012-03-01 | E-GEOD-35017 | biostudies-arrayexpress

Neurospora crassa strain:4258 T(IV;VI)P347

Project description:Neurospora crassa 4258 T(IV;VI)P347 Resequencing

| PRJNA346021 | ENA

Neurospora crassa strain:3208 T(I->VII)P7442 mo

Project description:Neurospora crassa 3208 T(I->VII)P7442 mo Resequencing

| PRJNA346005 | ENA

Neurospora crassa strain:3209 T(I->VII)P7442 mo

Project description:Neurospora crassa 3209 T(I->VII)P7442 mo Resequencing

| PRJNA346006 | ENA

Characterization of Neurospora crassa ghh (NCU06095) mutant strains

Project description:To determine the genes directly and indirectly under the control of the Grainy-head homolog (GHH) transcription factor in Neurospora crassa

2012-03-01 | GSE35017 | GEO

Gene expression levels in three Neurospora species reveal genes crucial for sexual reproduction in Neurospora crassa

Project description:Many fungi form complex three-dimensional fruiting bodies, within which the meiotic machinery for sexual spore production has been considered to be largely conserved over evolutionary time. Indeed, much of what we know about meiosis in plant and animal taxa has been deeply informed by studies of meiosis in Saccharomyces and Neurospora. Nevertheless, the genetic basis of fruiting body development and its regulation in relation to meiosis in fungi is barely known, even within the best studied multicellular fungal model Neurospora crassa. We characterized morphological development and genome-wide transcriptomics in the closely related species Neurospora crassa, Neurospora tetrasperma, and Neurospora discreta, across eight stages of sexual development. Despite diverse life histories within the genus, all three species produce vase-shaped perithecia. Transcriptome sequencing provided gene expression levels of 2479 orthologous genes among all three species. Expression of key meiosis genes and sporulation genes, corresponded to developmental differences among these Neurospora species during sexual development. Screening N. crassa knockout crosses of genes selected for their expression differences across species, eight genes, whose functions were previously unknown, are found to be critical for the successful formation of perithecia. The absence of these genes in mutant crosses resulted in either no perithecium formation or in arrested development at an early stage. Our results provide insight into the genetic basis of Neurospora sexual reproduction, which is also of great importance with regard to other multicelluar ascomycetes, including fungal pathogens closely related to Neurospora in the Sordariomycetes, such as Fusarium spp, Magnaporthe oryzae, and Nectria haematococca mRNA were sampled and compared from eight time points across sexual reproduction in three Neurospora species

2014-12-12 | E-GEOD-60255 | biostudies-arrayexpress

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