Project description:In this study, we report the complete mitochondrial genome of Hirsutella rhossiliensis (Ophiocordycipitaceae, Hypocreales, Ascomycota). We construct the mitochondrial DNA genome organization of 62?483?bp in length of H. rhossiliensis by using the whole-genome resequencing method. Conserved genes including the large and small rRNA subunits, 26 tRNA and 14 protein-coding genes are identified. These protein-coding genes utilize ATG, GTG or TTG as initiation codons and TAA or TAG as termination codons. Moreover, we detect 10 group I introns and one unclassified intron in six genes (rnl, cob, cox1, cox3, nad1 and nad5) encoding ORFs of ribosomal protein S3 and GIY-YIG/LAGLIDADG endonucleases or hypothetical proteins. This mitochondrial genome will be useful in understanding the distribution and genetic diversity of this species.