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Cercospora zeina strain:CMW25467

ABSTRACT: Cercospora zeina strain:CMW25467 Genome sequencing and assembly

PROVIDER: PRJNA355276 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	MVDW01.dat.gz	Other
	MVDW01.fasta.gz	Fasta.gz
	MVDW01.master.dat	Other
	MVDW01.dat.gz	Other

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Cercospora zeina

Project description:Cercospora zeina overview

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Project description:Gray leaf spot (GLS) disease of maize can be caused by either of two sibling fungal species Cercospora zeina or Cercospora zeae-maydis. These species differ in geographical distribution, for example to date only C. zeina is associated with GLS in African countries, such as South Africa. Maize inbred line B73, which is susceptible to GLS, was planted in the field, and subjected to natural infection with C. zeina. Samples were collected from lower leaves with substantial GLS lesions and younger upper leaves of the same plants with very few immature GLS lesions. The first aim of the experiment was to determine which maize genes are induced in response to C. zeina infection. The second aim was to identify C. zeina genes expressed in planta during a compatible interaction. The third aim was to determine whether the C. zeina cercosporin biosynthetic (CTB) genes are expressed in planta. C. zeina fails to produce cercosporin in vitro in contrast to C. zeae-maydis. Cercosporin is a phytotoxin that is thought to play a role in pathogenicity of several Cercospora spp., however its role in the pathogenicity strategy of C. zeina is currently under investigation.

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RNA-Seq analysis of maize lines resistant and susceptible to Cercospora zeina, causal organism of Grey leafspot disease

Project description:Purpose: The aim was to compare the transcriptome (using RNASeq) of a Cercospora zeina-resistant line (RIL387) and a Cercospora zeina susceptible line (RIL165), following inoculation by Cercospora zeina, in order to identify the defense responses associated with each line. Methods: Plants were grown in rows in three randomised blocks. Natural infection with Cercospora zeina was allowed to take place. Infected leves were harvested at the R5-early dent stage. Three leaves (one per plant) were harvested per block and pooled to make up one biological rep. One biological rep was harvested per block for each line. RNA was extracted and sequenced using Illumina. Sequencing reads were mapped to the B73 reference genome and were analysed using the Tuxedo suite of tools (Top hat, Cufflinks, Cuffmerge and Cuffdiff). Results: 5349 genes were differentially expressed between RIL165 and RIL387. In order to dissect responses specific to RIL165 and RIL387, 2394 genes with Log2FC ≤-1 were defined as having higher expression in RIL165 compared to RIL387 and 1393 genes with a Log2FC ≥1 as having increased expression in RIL387 compared to RIL165. Nine genes were validated using RT-qPCR. Four reference genes were included in the RT-qPCR analysis. Differentially expressed genes were further analysed for GO enrichment and pathway representation. Conclusions: Our RNA-Seq results show that a resistant and susceptible line repond differently to infection with Cercospora zeina, as differentially expressed genes were identified between the two lines.

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Cercospora zeina

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