ABSTRACT: Mycorrhizal fungal microbial communities from INRA, France, for Deep Transcriptomics studies - Tulasnella calospora - Serapias vomeracea metatranscriptome
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Serapias vomeracea-Tulasnella calospora mycorrhizal protocorms compared to asymbiotic protocorms . Protocorms were harvested at stage P2 and used for RNA extraction. Reads of 150bp were generated and aligned to a de novo transcriptome assembly from Serapias vomeracea using CLC Genomics Workbench 9.
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Tulasnella calospora mycorrhizal protocorms compared to free-living mycelium . Protocorms and control mycelium were harvested after 30 days and used for RNA extraction. Reads of 2X100bp were generated and aligned to Tulasnella calospora transcripts (http://genome.jgi-psf.org/Tulca1) using CLC Genomics Workbench 6. mRNA profiles from Tulasnella calospora mycorrhizal protocorms and free-living mycelium were generated by paired-end (2x100bp) Illumina HiSeq2000 sequencing. Three biological replicates were sequenced for mycorrhizal and mycelium samples.
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Tulasnella calospora mycorrhizal protocorms compared to free-living mycelium . Protocorms and control mycelium were harvested after 30 days and used for RNA extraction. Reads of 2X100bp were generated and aligned to Tulasnella calospora transcripts (http://genome.jgi-psf.org/Tulca1) using CLC Genomics Workbench 6.
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Tulasnella calospora mycorrhizal protocorms at stage P2 compared to free-living mycelium grown on Modified Melin-Norkrans medium with two different N sources, ammonium (AL13M) and glutamine (AL1 G) . Protocorms were harvested at stage P2 and mycelium after 15 days and used for RNA extraction. Reads of 150bp were generated and aligned to Tulasnella calospora transcripts (http://genome.jgi-psf.org/Tulca1) using CLC Genomics Workbench 9.
