Project description:To overcome the inhibition caused by the fermentation supernatant in the late fermentation stage of docosahexaenoic acid (DHA)-producing Crypthecodinium cohnii, fermentation supernatant-based adaptive laboratory evolution (FS-ALE) was conducted. The cell growth and DHA productivity of the evolved strain (FS280) obtained after 280 adaptive cycles corresponding to 840 days of evolution were increased by 161.87% and 311.23%, respectively, at 72 h under stress conditions and increased by 19.87% and 51.79% without any stress compared with the starting strain, demonstrating the effectiveness of FS-ALE.

2021-11-27 | PXD030028 | iProX

Staphylococcus cohnii subsp. cohnii strain:Barensis

Project description:Staphylococcus cohnii subsp. cohnii strain:Barensis Genome sequencing and assembly

| PRJNA607668 | ENA

Staphylococcus cohnii subsp. cohnii strain:G22B2

Project description:Staphylococcus cohnii subsp. cohnii strain:G22B2 Genome sequencing and assembly

| PRJNA275680 | ENA

Staphylococcus cohnii subsp. cohnii

Project description:Staphylococcus cohnii subsp. cohnii isolated from human patients

| PRJNA279286 | ENA

Staphylococcus cohnii subsp. cohnii

Project description:Staphylococcus cohnii subsp. cohnii NBRC 109713 genome sequencing project

| PRJDB1638 | ENA

Genetic Engineering of Crypthecodinium cohnii to Increase Growth and Lipid Accumulation.

Project description:In this study, we evaluated suitable selected markers and optimized transformation protocols to develop a new genetic transformation methodology for DHA-producing Crypthecodinium cohnii. Additionally, ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), potentially involved in CO2 fixation under autotrophic conditions, was selected as the target for construction of a gene knockdown mutant. Our results show that the constructs were successfully inserted into the C. cohnii chromosome by homologous recombination. Comparative analysis showed that deletion of the RuBisCO gene promoted cell growth and increased the lipid content of C. cohnii under heterotrophic conditions compared with those of the wild-type. The liquid chromatography-mass spectrometry (LC-MS) based metabolomic analysis showed that the metabolites involved in energy metabolism were upregulated, suggesting that the deletion of the RuBisCO gene may contribute to the re-direction of more carbon or energy toward growth and lipid accumulation under heterotrophic conditions.

| S-EPMC5868476 | biostudies-literature

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