Project description:Transcriptomic analysis of C. difficile strain 630 subjected to a clinically relevant heat stress (41°C ) and compared to unstressed cells (37°C ). Heat stress was induced in biological triplicate broth cultures in the early exponential phase (D650nm=0.3) of growth and cells were harvested at late log phase (D650 = 1.1). Goal was to determine effects of mild heat stress on gene expression.
Project description:Transcriptomic analysis of C. difficile strain 630 subjected to a clinically relevant heat stress (41M-BM-0C ) and compared to unstressed cells (37M-BM-0C ). Heat stress was induced in biological triplicate broth cultures in the early exponential phase (D650nm=0.3) of growth and cells were harvested at late log phase (D650 = 1.1). Goal was to determine effects of mild heat stress on gene expression. Two condition experiment, heat stressed vs. control. Biological replicates: 3 control replicates, 3 heat stressed replicates, with dye flip
Project description:We designed an experimental setup to investigate the transcriptomic and proteomic responses of the hyperthermophilic archaeon Pyrococcus furiosus to heat and cold shock. P. furiosus is a model organism for studying microbial adaptation to extreme environments, including deep-sea hydrothermal vents with temperature gradients ranging from 1°C to 400°C. We aimed to simulate critical conditions where P. furiosus cannot grow and to examine the immediate response to thermal stress as well as the recovery process.
Project description:Heat-evolved Symbiodiniaceae can improve the physiological performances of their coral host under heat stress, but their gene expression responses to heat remained unknown. We explore here the transcriptomic basis of differential thermal stress responses between in hospite wild-type and heat-evolved Cladocopium proliferum strains and their coral host Platygyra daedealea.
2024-03-01 | GSE256269 | GEO
Project description:Transcriptomic analysis reveals decreased immunity of Asian yellow pond turtle under heat stress
