Iguana iguana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA376378 | ENA |
REPOSITORIES: ENA
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Project description:The morphogen Sonic H edgehog governs a wide range of developmental processes. The zebrafish genetic mutant iguana has vascular stability defects due to decreased Shh signaling. Using iguana mutant embryos and embryos treated with the Hedgehog pathway inhibitor cyclopamine, we conducted a microarray to determine genes that are specifically regulated by Shh signaling, and that might mediate vascular stability. We populate a list of 40 genes to have significantly altered expression in both conditions. Using in situ hybridization and quantitative real-time PCR, we verify the expression changes seen in a subset of genes from the list and determine their localization during embryonic development. We then assay the functional relevance of one of the array hits, the cell-cycle regulator pim1, which was upregulated on the microarray. By overexpressing pim1, we observe a loss of vascular stability, similar to that of iguana mutants. Furthermore, chemical inhibition of pim1 in iguana mutant embryos or cyclopamine treated embryos rescues vascular stability. We conclude that the microarray identified a set of genes that are differentially expressed in two distinct modes of Shh signaling interference. Furthermore, this set of genes contains a high proportion of factors potentially involved in vascular stabilization. The identification of these genes is the first step in defining the molecular mechanism by which Shh promotes vascular stability. 3 biological cyclopamine treated samples plus 3 biological DMSO treated controls, plus 3 biological replicates of iguana mutants plus 3 wild type sibling controls, all collected at 30 hpf
2014-06-30 | E-GEOD-48335 | biostudies-arrayexpress
Project description:The morphogen Sonic H edgehog governs a wide range of developmental processes. The zebrafish genetic mutant iguana has vascular stability defects due to decreased Shh signaling. Using iguana mutant embryos and embryos treated with the Hedgehog pathway inhibitor cyclopamine, we conducted a microarray to determine genes that are specifically regulated by Shh signaling, and that might mediate vascular stability. We populate a list of 40 genes to have significantly altered expression in both conditions. Using in situ hybridization and quantitative real-time PCR, we verify the expression changes seen in a subset of genes from the list and determine their localization during embryonic development. We then assay the functional relevance of one of the array hits, the cell-cycle regulator pim1, which was upregulated on the microarray. By overexpressing pim1, we observe a loss of vascular stability, similar to that of iguana mutants. Furthermore, chemical inhibition of pim1 in iguana mutant embryos or cyclopamine treated embryos rescues vascular stability. We conclude that the microarray identified a set of genes that are differentially expressed in two distinct modes of Shh signaling interference. Furthermore, this set of genes contains a high proportion of factors potentially involved in vascular stabilization. The identification of these genes is the first step in defining the molecular mechanism by which Shh promotes vascular stability.
2014-06-30 | GSE48335 | GEO
Project description:Thermal proteome profiling data for SW480 colon cancer cells treated with either the ALDH1B1 inhibitor IGUANA-1 or DMSO vehicle alone.
2022-02-11 | MSV000088824 | MassIVE
Project description:Communication between individuals via molecules, termed chemosignaling, is widespread among animal and plant species. However, we lack knowledge on the specific functions of the substances involved for most of such systems. The femoral gland is an organ secreting a waxy substance, which is involved in chemical communication in lizards. While the lipids and volatile substances secreted by the femoral glands have been investigated in several biochemical studies, the protein composition and functions of secretions are completely unknown. Applying a proteomic approach, we provide the first attempt to comprehensively characterize the protein composition of femoral gland secretions from the Galápagos marine iguana. Using samples from several organs, the marine iguana proteome was assembled by next generation sequencing and mass spectrometry, resulting in 7,513 proteins; 4305 proteins were present in the femoral gland, including keratins, small serum proteins and fatty acid-binding proteins. Surprisingly, no proteins with obvious roles in partner recognition or inter-species communication could be identified. However, we found several proteins with direct associations to the innate immune system, such as lysozyme C, antileukoproteinase (ALP), pulmonary surfactant protein (SFTPD) and galectin (LGALS1), suggesting the femoral glands function as an important barrier to infection. The identification of previously unrecognized effective anti-microbial peptides from the femoral gland show similar activities against E.coli and B. subtilis compared to oncocin. This proteomics dataset is a valuable resource for future functional protein analysis and demonstrates that femoral glands secretions covers also functions of the innate immune system.
2020-06-29 | PXD018909 | Pride
Project description:Comparative analysis of genes regulated by Dzip1/iguana and Hedgehog in zebrafish
| PRJNA209714 | ENA