Saccharomyces cerevisiae
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA377576 | ENA |
REPOSITORIES: ENA
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Project description:Forkhead transcription factor FHL1 gene was required for replicative lifespan as well as cell proliferation in yeast. In this study, to see how Fhl1p determines the lifespan, we performed a DNA microarray analysis of heterozygous diploid strain deleted for FHL1 and, from the Fhl1p-target genes, screened for a lifespan-related gene. Transcriptomic profiles showed large increases and moderate decreases in transcripts in the fhl1/FHL1 mutant. Pathway analysis suggested upregulation of most of carbon metabolisms (glycolysis, gluconeogenesis, and TCA cycle) and downregulation of translation (ribosomal proteins and translation factors) in the FHL1-deficient mutant. We found a remarkable downregulation of a set of phosphate metabolism genes and ribonucleotide reductase large subunit genes, RNR1 and RNR3. We demonstrated that Fhl1p regulates RNR1 gene transcription to maintain dNTP levels, modulating longevity against replication stress and genomic instability.
2017-03-02 | GSE95591 | GEO
Project description:Affy data from WT, FHL1 deleted and FHL1,IFH1 double deleted strains.
| PRJNA91789 | ENA
Project description:Effect of FLO8 or MSS11 deletion and -overexpression on yeast transcript profiles compared to wild type in laboratory yeast strains Σ1278b and S288c.
2011-09-21 | GSE17716 | GEO
Project description:Yeast deletion pool Barcode-sequencing
| PRJNA391272 | ENA
Project description:Affymetrix experiment performed on RNA isolated from Wild type, FHL1 deleted and FHL1,IFH1 double deleted strains. Data aquired in duplicate.,In S. cerevisiae the mRNAs from the 138 ribosomal protein (RP) genes are amongst the most abundant in the cell, and their transcription is regulated tightly so that they are the most prominent cluster in most transcriptome experiments. It has recently been observed that the proteins Fhl1p and Ifh1p are found almost exclusively at RP genes (Lee et al., Science, 298, 799-804, 2002;Jorgensen et al., Genes Dev, 18, 2491-2505 2004; Schawalder et al Nature in press; Rudra et al , EMBO J. submitted), and data suggests they are the true transcription factors.,This experiment utilizes Affymetrix arrays to measure the level of all mRNAs in cells with a deletion of FHL1 or of both FHL1 and IFH1, compared to the wild type strain, W303. Such mutant cells are viable but grow very slowly (Hermann-Le Denmat S. et al., Mol Cell Biol, 14, 2905-2913, 1994; Cherel and Thuriaux, Yeast, 11, 261-270, 1995). Total RNA was made from log phase cells, amplified according to conventional methods, and hybridized to the array. (Since the total RNA/cell of the mutants is only a fifth of the wt, each mutant required five times the number of cells to provide the same amount of RNA.) The experiment was carried out in duplicate and the six tables provide the raw data from the wt, W303, delFHL1, and delFHL1, delIFH1 strains.,Although we expected that the RP genes would be substantially reduced in these mutant cells, they are only slightly less abundant than normal in comparison to all other mRNAs! The interesting result is that the cells respond to a severe deficiency of ribosomes by reducing the level of nearly all mRNAs to match the capacity of the translational apparatus.
2007-07-07 | E-GEOD-2096 | biostudies-arrayexpress
Project description:Analysis of systemic genome instability in heterozygous yeast diploids
| PRJNA321105 | ENA