Mesomycoplasma hyopneumoniae strain:11 | isolate:MAM1238001_11
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA378403 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| MWWN01.dat.gz | Other | |||
| MWWN01.fasta.gz | Fasta.gz | |||
| MWWN01.master.dat | Other | |||
| SRR5320242_1.fastq.gz | Fastqsanger.gz | |||
| SRR5320242_2.fastq.gz | Fastqsanger.gz |
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Project description:Mycoplasma hyopneumoniae, the causative agent of swine enzootic pneumonia, colonizes the cilia of swine lungs, causing ciliostasis and cell death. Mycoplasma hyopneumoniae is a component of the porcine respiratory disease complex (PRDC) and is especially problematic for the finishing swine industry, causing the loss of hundreds of millions of dollars in farm revenues worldwide. For successful infection, M. hyopneumoniae must effectively resist oxidative stresses due to the release of oxidative compounds from neutrophils and macrophages during the host’s immune response. However, the mechanism M. hyopneumoniae uses to avert the host response is still unclear. To gain a better understanding of the transcriptional responses of M. hyopneumoniae under oxidative stress, cultures were grown to early exponential phase and exposed to 0.5% percent hydrogen peroxide for 15 minutes. RNA samples from these cultures were collected and compared to RNA samples from control cultures using two-color PCR-based M. hyopneumoniae microarrays. This study revealed significant down-regulation of important glycolytic pathway genes and gene transcription proteins, as well as a protein known to activate oxidative stressor cascades in neutrophils. This study has also contained significantly differentially expressed genes common to other environmental stress responses, and merits further study of universal stress response genes of M. hyopneumoniae. Keywords: Mycoplasma hyopneumoniae, RNA microarray
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Project description:Mycoplasma hyopneumoniae is the causative agent of porcine enzootic pneumonia and a major factor in the porcine respiratory disease complex. A clear understanding of the mechanisms of pathogenesis does not exist although it is clear that M. hyopneumoniae adheres to porcine ciliated epithelium by action of a protein called P97. Previous studies have shown variation in the gene encoding the P97cilium adhesin within different strains of M. hyopneumoniae, but the extent of genetic variation among field strains across the genome is not known. Since M. hyopneumoniae is a worldwide problem, it is reasonable to expect that a wide range of genetic variability may exist given all of the different breed and housing conditions. This variation may impact the overall virulence of a single strain. Using microarray technology, this study examined potential variation of fourteen field strains in comparison to strain 232 on which the array was based. Genomic DNA was obtained, amplified with TempliPhi™, and labeled indirectly with Alexa dyes. Post genomic hybridization, the arrays were scanned and data analyzed using a linear statistical model. Results indicate that genetic variation could be detected in all fourteen field strains but across different loci, suggesting that variation occurs throughout the genome. Fifty-nine percent of the variable loci were hypothetical genes. Twenty-two percent of the lipoprotein genes showed variation in at least one field strain. A permutation test identified a location in M. hyopneumoniae genome where spatial clustering of variability between the field strains and strain 232 exists. Keywords: CGH, Mycoplasma Hyopneumoniae
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Project description:Kamminga2017 - Metabolic model of Mycoplasma hyopneumoniae growth
This model is described in the article:
Metabolic modeling of energy
balances in Mycoplasma hyopneumoniae shows that pyruvate
addition increases growth rate.
Kamminga T, Slagman SJ, Bijlsma JJE,
Martins Dos Santos VAP, Suarez-Diez M, Schaap PJ.
Biotechnol. Bioeng. 2017 Jun; :
Abstract:
Mycoplasma hyopneumoniae is cultured on large-scale to
produce antigen for inactivated whole-cell vaccines against
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requirements of this minimal bacterium and the low growth rate
make it challenging to reach sufficient biomass yield for
antigen production. In this study, we sequenced the genome of
M. hyopneumoniae strain 11 and constructed a high quality
constraint-based genome-scale metabolic model of 284 chemical
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in a standard M. hyopneumoniae cultivation was used for
non-growth associated maintenance and only 16% of cellular
energy was used for growth and growth associated maintenance.
Following a cycle of model-driven experimentation in dedicated
fermentation experiments, we were able to increase the fraction
of cellular energy used for growth through pyruvate addition to
the medium. This increase in turn led to an increase in growth
rate and a 2.3 times increase in the total biomass
concentration reached after 3-4 days of fermentation, enhancing
the productivity of the overall process. The model presented
provides a solid basis to understand and further improve M.
hyopneumoniae fermentation processes. Biotechnol. Bioeng.
2017;9999: 1-9. © 2017 Wiley Periodicals, Inc.
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2017-08-08 | MODEL1704250001 | BioModels
Project description:Mycoplasma hyopneumoniae 168 genome sequencing
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