Project description:Lasiodiplodia theobromae is one of the causal agents of Grapevine trunk diseases, which becomes a tremendous threat to the grapevine production worldwide. Plant pathogens secrete diverse effectors to suppress host immune responses or regulate the host metabolism to promote diseases. Our results suggest that L. theobromae LtCre1 targets host VvRHIP1 to manipulate the sugar signaling pathway, through disrupting the association of VvRHIP1 and VvRGS1 complex.
Project description:Botryosphaeriaceae is an important fungal family that can colonize a wide range of woody hosts including economically important plants and cause serious diseases worldwide. To uncover the virulence variation in Lasiodiplodia theobromae, we sequenced the transcriptome of Las1 in L. theobromae inoculated with the stems of V.vinifera using dual-RNAseq method. We found a lot of expanded genes were induced after inoculated with grapevine compared with the transcriptome of Las1 that was maintained in PDA under the same condition.
Project description:A multi-omics approach was used to understand how temperature affects the molecular mechanisms of pathogenicity of Lasiodiplodia theobromae at different temperatures. The transcriptome (Illumina TruSeq) of LA-SOL3 strain grown at 25 °C and 37 °C was analysed.
Project description:Identification and characterization of potato long noncoding RNAs responsive to Pectobacterium carotovorum subspecies brasiliense infection
Project description:We report the use of a Dual RNA-Seq approach to investigate the interaction of grapevine with the Botryosphaeria dieback agent L. theobromae. The aim of this work was to (1) identify pathogenicity factors produced by L. theobromae, and (2) determine the V. vinifera defence response to the pathogen.
Project description:HCV infection requires both virus and host factors, including endogenous genes, a large proportion of which are noncoding RNAs (ncRNAs). The identification and mechanistic elucidation of these stably and conservatively expressed RNAs will shed light on HCV gene diagnosis and therapy. Nevertheless, most studied ncRNAs in HCV are microRNAs, with numerous other types of ncRNAs being neglected, such as long noncoding RNAs (lncRNAs) or small nucleolar RNAs (snoRNAs). Here, using two different hepatoma cell lines, we performed small RNA sequencing and analyzed the differential expression of microRNAs and snoRNAs, which provide data for further functional validation of snoRNAs and microRNAs during HCV infection.
