Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: Exosome-mediated direct conversion of human fibroblasts into neural progenitor-like cells [WTS]

PROVIDER: PRJNA384022 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5470292_1.fastq.gz	Fastqsanger.gz
	SRR5470292_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Exosome-mediated direct conversion of human fibroblasts into neural progenitor-like cells

Project description:Exosome-mediated direct conversion of human fibroblasts into neural progenitor-like cells

| PRJNA384018 | ENA

Homo sapiens

Project description:Exosome-mediated direct conversion of human fibroblasts into neural progenitor-like cells [MBD-seq]

| PRJNA384025 | ENA

Homo sapiens

Project description:Exosome-mediated direct conversion of human fibroblasts into neural progenitor-like cells [ncRNA-Seq]

| PRJNA384023 | ENA

Homo sapiens

Project description:Exosome-mediated direct conversion of human fibroblasts into neural progenitor-like cells [RNA-Seq]

| PRJNA384024 | ENA

The global gene expression pattern between human fibroblasts and human neural stem cells

Project description:Recent advances in direct reprogramming using cell type-specific transcription factors provide an unprecedented opportunity for rapid generation of desired human cell types from easily accessible tissues. However, due to the diversity of conversion factors that facilitate the process, an arduous screening step is inevitable to find the appropriate combination(s). Here, we show that under chemically defined conditions minimal pluripotency factors are sufficient to directly reprogram human fibroblasts into stably self-renewing neural progenitor/stem cells (NSCs), but without passing through a pluripotent intermediate stage. These NSCs can be expanded and propagated in vitro without losing their potential to differentiate into various neuronal subtypes and glia. Our direct reprogramming strategy represents a simple and advanced paradigm of direct conversion that will provide an unlimited source of human neural cells for cell therapy, disease modeling, and drug screening. We used microarray to compare the global gene expression pattern between human fibroblasts and human neural epitheliums from human ESCs or directly from fibroblasts. We cultured cells and harvested them and then extracted total RNA for microarray.

2013-01-16 | E-GEOD-38045 | biostudies-arrayexpress