Project description:Next Generation Sequencing Facilitates Quantitative Analysis of HL-60 cells transduced with control or JMJD3-expression vector, and HL-60 parental and JMJD3 knockout cells
Project description:RNA-seq was performed to access the differences in transcriptome profiling of neutrophil-like HL-60 cells pre- and 5 days post-differentiation and upon BAR domain protein knockout (post-differentiation). Samples were collected before or after 5 days of differentiation with 1.5% DMSO for wild-type HL-60 cells as well as 5 days post-differentiation for wild-type and Snx33 knockout HL-60 cells.
Project description:Homo sapiens fresh whole blood was infected with Candida tropicalis. RNA-pool of both species extracted at 0min (control), 15, 30, 60, 120, 240 min. Samples are rRNA depleted. Measurement of Candida tropicalis gene expression.
