Project description:To determine the mechanisms of improved toxic metal tolerance of A. arenosa inoculated with Mucor sp. whole gemone microarray profiling was employed. Metal toxicity altered the expression of 5169 probe sets; 2861 genes were up-regulated and 2308 down-regulated. Inoculation with the fungi induced changes in the expression of 899 probes from the above presented data set; 492 genes were up-regulated and 314 were down-regulated. The expression of 93 genes (63 and 30, up and down-regulated respectively) was affected by both treatments (Fig. 5A-B). Genes differentially expressed in E+ plants vs E- from mine dump substrate were subject to GO analysis (gene ontology) to identify enriched terms with a cut-off rate of 0.01. The majority of the genes upregulated belonged to ROS metabolism, response to biotic and abiotic stimuli, response to ion starvation and ET biosynthesis and signaling functional categories. Among down-regulated transcripts GO analysis revealed a significant enrichment in the regulation of anthocyanin biosynthesis term.
Project description:Hybridization to Affymetrix tiling array Ath 1.0R performed using gDNA from Arabidopsis arenosa var. Care-1, Arabidopsis thaliana var 4x Ler, Arabidopsis suecica var. Sue-1, and F1 hybrids between A. thaliana var 4x Ler and A. arenosa var. Care-1
