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Saccharomyces cerevisiae

ABSTRACT: Whole genome ChIP-seq of histone H3 threonine 11 phosphorylation in Saccharomyces cerevisiae

PROVIDER: PRJNA392121 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5761570_1.fastq.gz	Fastqsanger.gz
	SRR5761570_2.fastq.gz	Fastqsanger.gz
	SRR5761580_1.fastq.gz	Fastqsanger.gz
	SRR5761580_2.fastq.gz	Fastqsanger.gz
	SRR5761571_1.fastq.gz	Fastqsanger.gz

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Whole genome ChIP-seq of histone H3 threonine 11 phosphorylation in Saccharomyces cerevisiae

Project description:We used ChIP-seq to determine the whole-genome enrichment of histone H3 threonine 11 phosphorylation (H3 T11ph) during Saccharomyces cerevisiae meiosis. S. cerevisiae SK1 cells were synchronized for meiotic entry and 3 and 4 hour meiotic samples were obtained. As H3 T11ph is dependent on the formation of meiotic double strand breaks (DSBs), a negative control ChIP-seq sample was obtained from a strain lacking DSBs (spo11-yf). Concurrently, ChIP-seq was carried out for histone H3 as a control for comparision.

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Histone H3 threonine 11 phosphorylation is catalyzed directly by the meiosis-specific kinase Mek1 and provides a molecular readout of Mek1 activity in vivo

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