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Drosophila melanogaster

ABSTRACT: MNase-seq after CBP inhibition in Drosophila S2 cells

PROVIDER: PRJNA392298 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5765600_1.fastq.gz	Fastqsanger.gz
	SRR5765600_2.fastq.gz	Fastqsanger.gz
	SRR5765601_1.fastq.gz	Fastqsanger.gz
	SRR5765601_2.fastq.gz	Fastqsanger.gz
	SRR5765602_1.fastq.gz	Fastqsanger.gz

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CBP occupancy in Drosophila S2 cells

Project description:CREB-binding protein (CBP, also known as nejire) is a transcriptional co-activator that is conserved in metazoans. We have generated CBP ChIP-seq data from Drosophila S2 cells and compared it to modENCODE data. This shows that CBP is bound at genomic sites with a wide range of functions. As expected, we find that CBP is bound at active promoters and enhancers. In addition, we find that the strongest CBP sites in the genome are found at Polycomb Response Elements embedded in histone H3 lysine 27 trimethylated (H3K27me3) chromatin, where they correlate with binding of the Pho repressive complex. Interestingly, we find that CBP also binds to most insulators in the genome. At a subset of these, CBP may regulate insulating activity, measured as the ability to prevent repressive H3K27 methylation from spreading into adjacent chromatin. ChIP seq in Drosophila S2 cells using two different antibodies against CBP (nejire), one raised in rabbit against amino acids 2540-3190 (CBP rb), and one raised in guinea-pig against amino acids 1-178 (CBP gp)

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Project description:GRO-seq in Drosophila melanogaster S2 cells

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Project description:ChIP-seq of Atrophin in Drosophila S2 cells

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CTCF/CP190 and ISWI dependent regulation of nucleosome occupancy [MNase-seq]

Project description:MNase-seq was performed in order to analyze changes in nucleosomal occupancy after depletion of CTCF/P190 and ISWI from Drosophila S2 cells MNase-seq from Drosophila S2 nuclei after CTCF/CP190 or ISWI-specific RNAi treatment

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