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Photorhabdus laumondii subsp. laumondii TTO1

ABSTRACT: DNA adenine methyltransferase (Dam) overexpression impairs Photorhabdus luminescens motility and virulence

PROVIDER: PRJNA392424 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5772130.fastq.gz	Fastqsanger.gz
	SRR5772131.fastq.gz	Fastqsanger.gz
	SRR5772132.fastq.gz	Fastqsanger.gz
	SRR5772127.fastq.gz	Fastqsanger.gz
	SRR5772128.fastq.gz	Fastqsanger.gz

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DNA adenine methyltransferase (Dam) overexpression impairs Photorhabdus luminescens motility and virulence

Project description:Dam, the most described bacterial DNA-methyltransferase, is widespread in gamma-proteobacteria. Dam DNA methylation can play a role in various genes expression and is involved in pathogenicity of several bacterial species. In the entomopathogenic bacterium Photorhabdus luminescens, a dam ortholog was identified. Overexpression of dam in P. luminescens did not impair growth ability in vitro. In contrast, compared to a control strain harboring an empty plasmid, a significant decrease in motility was observed in the dam-overexpressing strain. In addition, the dam-overexpressing P. luminescens strain showed a delayed virulence compared to that of the control strain after injection in larvae of the lepidopteran Spodoptera littoralis. These results reveal that Dam plays a major role during P. luminescens insect infection.

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Photorhabdus laumondii subsp. laumondii TTO1

Project description:Transcriptionnal analysis of a Photorhabdus luminescens TT01 variant, named VAR*, which is a colonial and phenotypic variant displaying delayed pathogenicity in the insect, Spodoptera littoralis.

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Project description:Gene expression of a Photorhabdus M-form variant relative to the wild type P-form cells

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Photorhabdus laumondii subsp. laumondii strain:HP88

Project description:Photorhabdus luminescens subsp. laumondii HP88 genome sequencing and assembly

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Transcriptionnal analysis of a Photorhabdus luminescens TT01 variant, named VAR*, which is a colonial and phenotypic variant displaying delayed pathogenicity in the insect, Spodoptera littoralis.

Project description:Photorhabdus luminescens lives in a mutualistic association with entomopathogenic nematodes and is pathogenic for insects. Variants of Photorhabdus frequently arise. VAR* is a colonial and phenotypic variant displaying delayed pathogenicity in the insect, Spodoptera littoralis. In this study, we evaluated the role of transcriptomic modulation in determining the phenotypic variation and delayed pathogenicity of VAR* with respect to the corresponding wild-type form, TT01alpha. A P. luminescens microarray was used to determine changes in transcript profiles in the exponential and stationary phase growth phase of VAR* and TT01alpha grown in LB broth.

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