Project description:Here, we define the VanT-QS regulon and explore the diversity and trajectory of traits under QS regulation in the fish pathogen Vibrio anguillarum through comparative transcriptomics of two wildtype strains and their corresponding mutants artificially locked in QS-on (ΔvanO) or QS-off (ΔvanT) states. We reveal that populations of one strain primarily employ a QS-off response, while the other strain mainly maneuvers within the QS-on spectrum. Further examination of our V. anguillarum collection revealed that ~15 % of the strains were QS-negative and measurements of a total of 9 QS-positive strains suggested diverse QS responses, which underlines an extensive diversity of QS delegations. We show that QS control a plethora of genes involved in processes such as central metabolism, biofilm, competence, T6SS and virulence in V. anguillarum. Interestingly, the low QS response strain had an enlarged QS regulon compared to the high QS response strain, suggesting a potential trade-off between engagement in QS and output of the commitment. Moreover, the combination of present and previous virulence trials coupled with QS response measurements indicate that the QS state is an important driver of virulence toward fish larvae in V. anguillarum. We propose that infections by mixed-strain communities spanning diverse QS strategies might utilize the animal host more efficiently. Furthermore, we emphasize the importance of applying whole-cell spike-in normalization to study gene expression of cell populations with differential total RNA content per cell.

2024-09-02 | GSE184853 | GEO

Edwardsiella anguillarum ET080813

Project description:Edwardsiella tarda 080813 genome project

| PRJNA39899 | ENA

Expression analysis of channel catfish anterior kidney at 48h post immersion challenge of vaccinated fish (28 days post vaccination with attenuated Edwardsiella ictaluri) with virulent E. ictaluri

Project description:To determine the global gene transcriptional changes in vaccinated channel catfish anterior kidney after immersion challenge with a virulent Edwardsiella ictaluri compared to sham vaccinated catfish control. The attenuated Edwardsiella ictaluri used for vaccination was Aquavac-ESC which was commercially available, the virulent strain of Edwardsiella ictaluri is the AL93-58 strain.

2011-10-05 | GSE32588 | GEO

Transcriptome analysis of the zebrafish embryonic host response to Edwardsiella tarda infection [experiment B]

Project description:Transcriptional profiling of the zebrafish embryonic host response to infection by injection of 200 CFUs of Edwardsiella tarda (strain FL6-60)

2011-10-01 | GSE28485 | GEO

Edwardsiella tarda strain:NCIMB2034

Project description:Edwardsiella tarda strain:NCIMB2034 Genome sequencing and assembly

| PRJNA357015 | ENA