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Sediment metagenome

ABSTRACT: Magnetotactic Bacteria Metagenome Project (MagMeta)

PROVIDER: PRJNA400260 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	PDZS01.dat.gz	Other
	PDZS01.fasta.gz	Fasta.gz
	PDZS01.master.dat	Other
	PDZT01.dat.gz	Other
	PDZT01.fasta.gz	Fasta.gz

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Identification of Iron-Inducible Genes in Magnetospirillum magneticum AMB-1 Non-Magnetic Mutant

Project description:Iron is limiting in the environment, bacteria respond to this deprivation by activating genes required for bacterial iron homeostasis. Transcriptional regulation in response to iron in Gram-negative bacteria is largely mediated by the ferric uptake regulator protein Fur, which in the presence of iron binds to a specific sequence in the promoter regions of genes under its control and acts as a repressor. Here we describe comparative global gene expression analysis using DNA microarray based on the whole genome sequence of the magnetotactic bacterium Magnetospirillum magneticum AMB-1 was conducted between wild type strain and a non-magnetic NMA61 mutant strain, generated by mini-Tn5 transposon mutagenesis which is incapable of assimilating iron to cytoplasm. No induction of the fur genes in NMA61 mutant strain was considered to be due to low intracellular iron concentration. In the iron-replete condition, among 4492 genes, 434 genes were down-regulated and 527 genes were up-regulated in the wild type strain. Among 434 genes down-regulated, 299 genes were not down-regulated in NMA61 mutant strain, indicating these genes are candidates of Fur-regulated. Keywords: Iron, magnetotactic bacteria

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