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Investigation of gene expression of Pseudomonas putida (NBAII RPF 9) under abiotic stress

ABSTRACT: Investigation of gene expression of Pseudomonas putida (NBAII RPF 9) under abiotic stress

PROVIDER: PRJNA400733 | ENA |

REPOSITORIES: ENA

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Investigation of gene expression of Pseudomonas putida (NBAII RPF 9) under abiotic stress [salt]

Project description:To undestand the mechanism involved in abitoic stress tolerance of P. Putida (NBAII-RPF 9) the bacterium was grown in liquid LB media overnight and further subjected to saline shock of (1M NaCl) for one hour seperately. The cultures were pelleted with centrifuged for total RNA. The RNA was hybridised in 8X15K Agilent array and image analysis carried out with Agilent Microarray scanner.

2017-08-31 | GSE103283 | GEO

Investigation of gene expression of Pseudomonas putida (NBAII RPF 9) under abiotic stress [heat]

Project description:To undestand the mechanism involved in abitoic stress tolerance of P. Putida (NBAII-RPF 9) the bacterium was grown in liquid LB media overnight and further subjected to heat shock at 45 degrees in incubated shaker for one hour seperately. The cultures were pelleted with centrifuged for total RNA. The RNA was hybridised in 8X15K Agilent array and image analysis carried out with Agilent Microarray scanner

2017-08-31 | GSE103282 | GEO

Investigation of gene expression of Pseudomonas putida (NBAII RPF 9) under abiotic stress

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-08-31 | GSE103284 | GEO

Pseudomonas putida

Project description:Investigation of gene expression of Pseudomonas putida (NBAII RPF 9) under abiotic stress [heat]

| PRJNA401900 | ENA

Pseudomonas putida

Project description:Investigation of gene expression of Pseudomonas putida (NBAII RPF 9) under abiotic stress [salt]

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Pseudomonas putida expression profiles at different stress conditions

Project description:The metabolically versatile Pseudomonas putida strain KT2440 is the first Gram-negative soil bacterium certified as a biosafety strain and is being used for applications in agriculture, biotechnology and bioremediation. P. putida has to cope in its niche with numerous abiotic stresses. The stress response to 4°C, pH 4.5, 0.8 M urea or 45 mM sodium benzoate, respectively, was analyzed by the global mRNA expression profile and screening for stress-intolerant Tn5 transposon mutants. In total we identified 49 gene regions to be differentially expressed and 32 genes in 22 operons to be indispensable for growth during exposure to one or the other abiotic stresses. We propose that stress is sensed by the outer membrane proteins OmlA and FepA and the inner membrane constituents PtsP, PhoPQ and CbrAB. The metabolic response is regulated by the cyo operon, the RelA/SpoT modulon, PcnB and VacB that control mRNA stability and BipA that exerts transcript-specific translational control. The adaptation of the membrane barrier, the uptake of phosphate, the maintenance of intracellular pH and redox status and the translational control of metabolism are the indispensable key mechanisms of the P. putida stress response. Keywords: functional genomics

2006-07-27 | GSE4048 | GEO

Gene expression analysis of Pseudomonas putida KT2440 Wild Type and its exopolysaccharide mutants under water stress

2013-12-23 | GSE53407 | GEO

Genome expression profiling of Chick pea under Abiotic Stress

Project description:Genome expression profiling of Chick pea under Abiotic Stress

2018-12-20 | GSE86807 | GEO

Gene expression analysis of Pseudomonas putida KT2440 Wild Type and its exopolysaccharide mutants under water stress

Project description:Alginate, a major exopolysaccharide (EPS) produced by P. putida, is known to create hydrated environments and alleviate the effect of water limitation. In addition to alginate, P. putida is capable of producing cellulose (bcs), putida exopolysaccharide a (pea), and putida exopolysaccharide b (peb). However, unlike alginate, not much is known about their roles under water limitation. Hence, in this study we examined the role of different EPS under water stress. To create environmentally realistic water stress conditions as observed in soil, we used Pressurized Porous Surface Model (PPSM). Our main hypothesis was that under water stress, absence of alginate would be compensated by the other EPS. To test our hypothesis, we investigated colony morphologies and whole genome transcriptomes of P. putida KT2440 WT and its mutants deficient in either alginate or all known EPS A custom-made Nimblegen (WI, USA) whole genome one-color oligonucleotide expression array (12x135K with 45-60 mer probes) of P. putida KT2440 was used to investigate effect of water stress on the differential expression of the whole genome. In this study Pseudomonas putida KT2440 wild type (WT) and two of its mutants deficient either in alginate (Alg-), or all known EPS (EPS-) production were used and grown under dry (water stress) and wet (without water stress) conditions. (Deleted genes in Alg-: PP1277-PP128; in EPS-: PP1277-1288 (alg) + PP2634-2638 (bcs) + PP3132-3142 (pea) + PP1795-1788 (peb)) (Nilsson et al., 2011).39. Nilsson, M., Chiang, W.C., Fazli, M., Gjermansen, M., Givskov, M., and Tolker-Nielsen, T. (2011) Influence of putative exopolysaccharide genes on Pseudomonas putida KT2440 biofilm stability. Environ Microbiol. 13 (5):1 357-1369

2013-12-23 | E-GEOD-53407 | biostudies-arrayexpress

Abiotic stress

Project description:To assess the reproducibility of the microarray analysis, we repeated the experiment three times with independently prepared total RNA. Keywords = abiotic stress Keywords: repeat sample

2005-03-02 | GSE2211 | GEO

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