Project description:Genome wide DNA methylation profiling of normal whole blood samples. The data consist of 100 samples with Illumina HumanMethylation450 BeadChip data. Bisulphite converted DNA from the 100 samples were hybridized to the Illumina HumanMethylation450 BeadChip
Project description:Genome-wide DNA methylation level was studied to identify the clustering of correlated DNA methylation at CpGs in whole blood. We used Illumina HumanMethylation450 BeadChip array to identify the clustering of correlated DNA methylation in whole blood from normal individuals from Baltimore Longitudinal Study of Aging (BLSA). Bisulphite converted DNA from normal individuals were hybridized to the Illumina Illumina HumanMethylation450 BeadChip arrays
Project description:Genome wide DNA methylation profiling of normal whole blood samples. The data consist of 43 samples with Illumina HumanMethylation450 BeadChip data. Bisulphite converted DNA from 43 of these samples were hybridized to the Illumina Infinium 450k Human Methylation Beadchip.
Project description:Aberrant methylation at the promoter region of tumor suppressors is frequently reported in NPC; however, genome-wide methylation changes have not been comprehensively investigated. Therefore, we systematically analyzed methylome data in 25 primary NPC tumors and non-tumor counterparts using Illumina HumanMethylation450 BeadChip. Bisulphite converted DNA from 50 samples were hybridized to the Illumina HumanMethylation450 BeadChip. Bisulphite pyrosequencing was used as an independent method to validate the BeadChip results.
Project description:Genome wide DNA methylation profiling of F-36P leukemic cells treated by arsenic disulfide, The Illumina HumanMethylation450 BeadChip was used to obtain DNA methylation profiles Bisulphite converted DNA from the 3 samples were hybridised to the Illumina HumanMethylation450 BeadChip
Project description:The gold standard bisulphite conversion technologies to study DNA methylation do not distinguish between 5mC and 5hmC, however new approaches to map 5hmC genome-wide have advanced rapidly, although it is unclear how the different methods compare in accurately calling 5hmC. In this study, we provide a comparative analysis on brain DNA using three 5hmC genome-wide approaches; namely whole-genome bisulphite/oxidative-bisulphite sequencing (WG Bis/OxBis-seq), Infinium HumanMethylation450 BeadChip arrays coupled with oxidative bisulphite (HM450K Bis/OxBis) and antibody-based immunoprecipitation and sequencing of hydroxymethylated DNA (hMeDIP-seq). We also compare the performance of these approaches in adult brain DNA, with a known high abundance of 5hmC, and cancer cell line LNCaP DNA, with cell lines known to have low levels of 5hmC.
