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MiRNA expression profile for NHLF after treatment with 10 ng/ml of IL-13

ABSTRACT: miRNA expression profile for NHLF after treatment with 10 ng/ml of IL-13

PROVIDER: PRJNA412089 | ENA |

REPOSITORIES: ENA

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miRNA expression profile for NHLF after treatment with 10 ng/ml of IL-13

Project description:To assess miRNA expression by IL-13 in NHLF, we carried out a miRNA analysis of NHLF treated with a with 10 ng/ml of IL-13 on 4 and 24 hr. We used the GeneChip® miRNA 4.0 Array

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RNA sequencing for enteroids treated with different concentrations of IL-17 (0 ng/ml. 1 ng/ml, 10 ng/ml and 100 ng/ml)

Project description:To evaluate the intestinal epithelial responses induced by IL-17, bulk RNA-sequencing (RNA-seq) was performed on the human small intestinal organoids (enteroids, n = 3) treated with different concentrations of IL-17 (0 ng/ml. 1 ng/ml, 10 ng/ml and 100 ng/ml) .

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RNA sequencing for enteroids treated with different concentrations of IL-17 (0 ng/ml. 1 ng/ml, 10 ng/ml and 100 ng/ml)

Project description:RNA sequencing for enteroids treated with different concentrations of IL-17 (0 ng/ml. 1 ng/ml, 10 ng/ml and 100 ng/ml)

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Genome-wide analysis of gene expression for NHLF after treatment with 10 ng/ml of IL-13

Project description:Genome-wide analysis of gene expression for NHLF after treatment with 10 ng/ml of IL-13

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Proteome of BaF3-mEpoR cells stimulated with 5 U/ml Epo, 10 ng/ml IL-3 or left untreated

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2022-09-28 | PXD035181 | Pride

Genome-wide analysis of coding, long noncoding RNA and miRNA expression for NHLF after treatment with 10 ng/ml of IL-13

Project description:Genome-wide analysis of coding, long noncoding RNA and miRNA expression for NHLF after treatment with 10 ng/ml of IL-13

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Genome-wide analysis of gene expression for NHLF after treatment with 10 ng/ml of IL-13

Project description:Normal human lung fibroblasts were stimulated by IL-13, and global gene expression profile was assessed by cDNA microarray

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Human airway epithelial cells co-treated with IL-4 and IL-13 for seven days.

Project description:Nasal basal epithelial cells from 6 healthy adult donors were grown in air-liquid interface (ALI) cultures for fourteen days. Cells were then treated with 10 ng/mL of IL-4 and 10 ng/mL of IL-13 for seven additional days. Next, cell lysates were used for low-input RNA-seq library preparation (Smart-seq2) and sequencing.

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IMR90 lung fibroblasts treated with IL-4 or IL-13

Project description:IL-4 and IL-13 are cytokines involved in type 2 immune responses involved in atopic asthma and other diseases. They have a partially overlapping set of cognate receptors whose roles are incompletely understood. Here we explore the effects of these cytokines on lung fibroblasts in order to assess the global similarities and contrasts in the genes whose expression their ligation modulates. IMR-90 lung fibroblast cells were cultured on matrigel or plastic and treated for 24 hours with IL-4 (10 ng/ml), IL-13 (10ng/ml), or TNFa (10 ng/ml) before harvest.

2013-12-31 | E-GEOD-47542 | biostudies-arrayexpress

Myeloid cells or lung fibroblasts treated with IL-4 or IL-13

Project description:IL-4 and IL-13 are cytokines involved in type 2 immune responses involved in atopic asthma and other diseases. They have a partially overlapping set of cognate receptors whose roles are incompletely understood. Here we explore the effects of these cytokines on various cells related to human lung disease in order to assess the global similarities and contrasts in the genes whose expression their ligation modulates. Monocytes, monocyte-differentiated (6 days, then 24h rest) macrophages, or normal primary lung fibroblasts were cultured on matrigel and treated for 6 or 24 hours with IL-4 (10 ng/ml), IL-13 (10ng/ml), IL-10 (20 ng/ml), TGFb (10 ng/ml), or dexamethasone (0.5 uM) before harvest.

2013-12-31 | E-GEOD-47538 | biostudies-arrayexpress

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