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Self-organized cerebral organoids with human specific features predict effective drugs to combat Zika virus infection

ABSTRACT: Self-organized cerebral organoids with human specific features predict effective drugs to combat Zika virus infection

PROVIDER: PRJNA412223 | ENA |

REPOSITORIES: ENA

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Self-organized cerebral organoids with human specific features predict effective drugs to combat Zika virus infection [RNA-seq]

Project description:The human cerebral cortex possess distinct structural and functional features that are not found in the lower species traditionally used to model brain development and disease. Accordingly, considerable attention has been placed on the development of methods to direct pluripotency stem cells to form human brain-like structures termed organoids. However, many organoid differentiation protocols are inefficient and display marked variability in their ability to recapitulate the three-dimensional architecture and course of neurogenesis in the developing human brain. Here, we report optimized organoid culture methods that efficiently and reliably produce cortical and basal ganglia structures similar to those in the human fetal brain in vivo. Neurons within the organoids are functional and exhibit network-like activities. We further demonstrate the utility of the organoid system for modeling the teratogenic effects of Zika virus on the developing brain and identifying new candidate receptors and therapeutic compounds that can mitigate its desructive actions.

2017-10-11 | GSE97919 | GEO

Self-organized cerebral organoids with human specific features predict effective drugs to combat Zika virus infection [expression array]

2017-10-11 | GSE97872 | GEO

Self-organized cerebral organoids with human specific features predict effective drugs to combat Zika virus infection

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-10-11 | GSE104279 | GEO

Self-Organized Cerebral Organoids with Human-Specific Features Predict Effective Drugs to Combat Zika Virus Infection.

Project description:The human cerebral cortex possesses distinct structural and functional features that are not found in the lower species traditionally used to model brain development and disease. Accordingly, considerable attention has been placed on the development of methods to direct pluripotent stem cells to form human brain-like structures termed organoids. However, many organoid differentiation protocols are inefficient and display marked variability in their ability to recapitulate the three-dimensional architecture and course of neurogenesis in the developing human brain. Here, we describe optimized organoid culture methods that efficiently and reliably produce cortical and basal ganglia structures similar to those in the human fetal brain in vivo. Neurons within the organoids are functional and exhibit network-like activities. We further demonstrate the utility of this organoid system for modeling the teratogenic effects of Zika virus on the developing brain and identifying more susceptibility receptors and therapeutic compounds that can mitigate its destructive actions.

| S-EPMC5637483 | biostudies-literature

Genetic analysis of a Zika Virus Envelope Glycosylation and Non-structural protein 4B P36 Double Mutant

Project description:Zika virus (ZIKV) is a mosquito-transmitted positive-sense RNA virus in the family Flaviviridae. Live attenuated vaccines have been successfully used to combat infection by flaviviruses, such as yellow fever and Japanese encephalitis viruses. A Zika virus harboring combined mutations in the envelope protein glycosylation site and in the nonstructural 4B protein amino acid 36 (ZE4B-36) was generated and assessed for stability, attenuation, and protection against infection. To determine the genetic stability of its RNA genome, ZE4B-36 was serially passaged in vitro in Vero cells. Virus harvested from passages (P)1 to P6 was subjected to next generation sequencing and downstream analysis to determine its nucleotide sequence variability. Specifically, single nucleotide variant analysis showed that the ZE4B-36 genome decreased its genetic diversity and resulted in a more stable nucleotide sequence. Thus, in addition to showing attenuation and protection, ZE4B-36 is a stable live attenuated virus that possesses characteristics important for a vaccine to combat Zika disease.

2021-12-31 | E-MTAB-9496 | biostudies-arrayexpress

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Project description:Self-organized cerebral organoids with human specific features predict effective drugs to combat Zika virus infection [expression array]

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Homo sapiens

Project description:Self-organized cerebral organoids with human specific features predict effective drugs to combat Zika virus infection [RNA-seq]

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COMBAT

Project description:The Carriage Of Multiresistant Bacteria After Travel (COMBAT) prospective cohort study

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Microarray analysis of MRC-5 cells infected with Zika virus strains

Project description:To better understand the critical drivers of Zika virus pathogenicity, we used microarray analysis to evaluate the host responses triggered by Zika virus infection in MRC-5 cells.

2021-03-23 | E-MTAB-10292 | biostudies-arrayexpress

RNA-seq transcript and gene data on zika exposed and zika naïve samples

Project description:RNA-seq count data at 3 timepoints was generated for Zika-exposed and Zika-naïve individuals in order to assess associated signatures

2019-04-17 | GSE129882 | GEO

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