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Expression data of human CD4+CD45RA+CCR7+ Naïve T cells

ABSTRACT: Expression data of human CD4+CD45RA+CCR7+ Naïve T cells

PROVIDER: PRJNA413796 | ENA |

REPOSITORIES: ENA

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Expression data of human CD4+CD45RA+CCR7+ Naïve T cells

Project description:Expression data of human CD4+CD45RA+CCR7+ Naïve T cells

| PRJNA413796 | ENA

Human Fetal and Adult Peripheral Naïve CD4+ T cells and CD4+CD25+ Treg cells

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Human peripheral blood CD4+ T cell subsets

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Human peripheral blood CD4+ T cell subsets

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Project description:Buffy coats from four independent human donors were enriched for CD4+ cells using MACS CD4 beads and LS columns. Cells were subsequently sorted into Naïve, Central Memory and Effector Memory using following markers: Naive: CD4+CD25–CD45RA+CCR7+; Central Memory: CD4+CD25–CD45RA–CCR7+; Effector Memory: CD4+CD25-CD45RA-CCR7-. Total RNA from these cell subsets was extracted and 100ng was used for Nanostring SPRINT run according to manufacturer's instructions. Overall aim of the experiment is assessing the expression level of human T helper cell miRNome.

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Human Fetal and Adult Peripheral Naïve CD4+ T cells and CD4+CD25+ Treg cells

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Gene expression in healthy and gene deficient human naïve CD4+ T cells

Project description:Ascertain the effects of disease-causing gene mutations on the differentiation status of human naïve CD4+ T cells in the setting of primary immunodeficiencies. Thus, do CD4+ T cells isolated according to a naïve surface phenotype (ie CD4+CD45RA+CCR7+) from healthy donors exhibit a similar gene expression profile as phenotpyically-matched cells isolated from individuals with defined primary immunodeficiencies caused by specific monogenic mutations.

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Expression data of human CD4+CD45RA+CCR7+ Naïve T cells

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Gene expression profiles of human CD45RA+CCR7-CD8+ T cells

Project description:Human CD8+ T cells are functionally heterogeneous and can be divided into distinct subsets according to CCR7 and CD45RA expression levels. Among the subsets, CCR7-CD45RA+ CD8+ T cells are considered to be terminally differentiated cells and designated as Temra. Temra show attenuated ability to proliferate and produce IFN-gamma in response to TCR stimulation, while Temra show improved function after IL-15 treatment. To clarify the transcriptional signatures induced by the stimulations, Temra were purified using flow cytometry, stimulated with IL-15 or with anti-CD3/CD28 (TCR stimulation), or cultured without stimulation for 2 days, and subjected to microarray analysis.

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