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RNA Sequencing Facilitates Quantitative Analysis of Transcriptomes of human mesenchymal stem cells differentiation from human embryonic stem cells and adult bone marrow mesenchymal stem cells

ABSTRACT: RNA Sequencing Facilitates Quantitative Analysis of Transcriptomes of human mesenchymal stem cells differentiation from human embryonic stem cells and adult bone marrow mesenchymal stem cells

PROVIDER: PRJNA413815 | ENA |

REPOSITORIES: ENA

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RNA Sequencing Facilitates Quantitative Analysis of Transcriptomes of human mesenchymal stem cells differentiation from human embryonic stem cells and adult bone marrow mesenchymal stem cells

Project description:Purpose: The goals of this study are to identify the process and the key transcription factors and signaling pathways governing differentiation through comparing the transcriptome profilings in hESC samples collected from day 0 to day 7 after mesenchymal stem cells differentiation with human adult bone marrow MSCs. Methods: mRNA profiles of human adult bone marrow MSCs and hESC samples collected from day 0 to day 7 after mesenchymal stem cells differentiation were generated by deep sequencing using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays Conclusions: Our study represents the first detailed analysis of mesenchymal stem cells transcriptomes from human embryonic stem cells, generated by RNA-seq technology. We provide a framework for comparative investigations of expression profiles and help illustrate the key signaling and transcription factors during hESCs differentiation towards MSCs.

2017-10-11 | GSE104784 | GEO

Exosomes of human minor salivary gland mesenchymal stem cells and adipose-derived stem cells LC-MS1

Project description:Proteome experiment was peformed on exosomes of human minor salivary gland mesenchymal stem cells and adipose-derived stem cells to find out the same points and difference of these two kinds of exosomes, which can hopefully give further guidance on further therapy research

2024-04-13 | PXD051432 | iProX

Quantitative transcriptome analysis of human embryonic stem cells, human mesenchymal stem cells and human musculoskeletal stem cells

Project description:Quantitative transcriptome analysis of human embryonic stem cells, human mesenchymal stem cells and human musculoskeletal stem cells

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Inferferon gamma induced compositional changes in human bone marrow derived mesenchymal stem/stromal cells

Project description:2D IDA protein quantitation of mesenchymal stem cells derived from bone marrow across five donors. A total of 10 2D LC-MS runs were performed, using cells both not stimulated and following a 20 hour treatment with interferon gamma.

2017-04-05 | MSV000080890 | MassIVE

Human bone-marrow-derived mesenchymal stem cells: control vs. cells undergoing chondrogenesis

Project description:Human bone-marrow-derived mesenchymal stem cells: control vs. cells undergoing chondrogenesis

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Quantitative proteomics of matrix stiffness-induced immune regulation in human mesenchymal stem cell

Project description:Quantitative proteomics of matrix stiffness-induced immune regulation in human mesenchymal stem cells

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Hypoxia-regulated miRNAs in Human Mesenchymal Stem Cells

Project description:Hypoxia-regulated miRNAs in Human Mesenchymal Stem Cells

| PRJNA517826 | ENA

Expression data from Human bone mesenchymal stem cells

Project description:Expression data from Human bone mesenchymal stem cells

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Primary human bone marrow-derived mesenchymal stem cells (MSC) (Chip_9965581030)

Project description:Mesenchymal stem cells are known to be recruited to the tumor and contribute to a pro-inflammatory environment. The aim of this experiment was to identify potential direct targets of hsa-miR-1246 in human bone marrow derived mesenchymal stem cells in the context of inflammation. For this purpose, miR-1246 mimic or miRIDIAN microRNA Mimic Negative Control #2 both purchased from GE Healthcare Dharmacon Inc. were transiently transfected with LipofectamineÂ® 2000 (Invitrogen AG) at a final concentration of 30nM into primary MSCs. Transfection time was 48h according to manufacturerâs protocol. Transfections were performed in biological triplicates each.

2016-12-30 | E-MTAB-5033 | biostudies-arrayexpress

Single cell RNA sequencing analysis of human embryonic stem cells, human embryoid bodies, human mesenchymal stem cells and human musculoskeletal stem cells

Project description:Single cell RNA sequencing analysis of human embryonic stem cells, human embryoid bodies, human mesenchymal stem cells and human musculoskeletal stem cells

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