Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

H9 human embryonic stem cells and Isthmic organizer (IsO) like cells

ABSTRACT: H9 human embryonic stem cells and Isthmic organizer (IsO) like cells

PROVIDER: PRJNA413986 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

H9 human embryonic stem cells and Isthmic organizer (IsO) like cells

Project description:Identify gene expression patterns of isthmic organizer (IsO) like cells derived from human embryonic stem cells.

2018-02-28 | GSE104847 | GEO

H9 human embryonic stem cells and Isthmic organizer (IsO) like cells

Project description:H9 human embryonic stem cells and Isthmic organizer (IsO) like cells

| PRJNA413986 | ENA

RNA-sequencing of LEUTX-inducible H9 human embryonic stem cells

Project description:RNA-sequencing of LEUTX-inducible H9 human embryonic stem cells

| PRJEB45411 | ENA

in vitro construction of an embryonic caudal organizer

Project description:in vitro construction of an embryonic caudal organizer

| PRJNA1129168 | ENA

Metabolomics Workbench ST000003 - GNPS - Metabolomic analysis of mouse embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells

Project description:mouse embryonic fibroblasts, embryonic stem cells, and induced pluripotent stem cells were compared via metabolomic analysis

2018-08-02 | MSV000082680 | GNPS

Chromatin immunoprecipitation of ectopic FLAG-tagged TCF7L1 followed by Mass Spectrome-try in H9-TCF7L1 Human Embryonic Stem Cells

Project description:TCF7L1 is a member of the T cell factor/Lymphoid enhancer factor (TCF/LEF) family of tran-scription factors that are part of the WNT/beta-CATENIN signaling pathway. TCF7L1 modulates transcription by interacting with other regulators on chromatin. TCF7L1 has been shown to be one of the key factors in maintaining pluripotency in human embryonic stem cells (ESCs). We previously demonstrated that the absence of TCF71 causes H9 hESCs to differentiate sponta-neously (Sierra et al., 2018 Development 145(4).pii:dev161075). Mechanistically, how TCF7L1 inhibits differentiation and keeps cells in the pluripotent state is not clear. Identifying transcrip-tional regulators on chromatin is a critical step to elucidating one of several molecular mecha-nisms controlled by TCF7L1. We previously developed a FLAG tagged TCF7L1 transgene that is controlled by a doxycycline-inducible TET-ON system and generated the H9-TCF7L1 line (Sier-ra et al., 2018 Development 145(4).pii:dev161075). Here we used the H9-TCF7L1 line and em-ployed the method called rapid immunoprecipitation (IP) mass spectrometry of endogenous pro-tein (RIME) (Mohammed et al., 2016 Nat Protoc 11(2):316-26) to identify TCF7L1-associated proteins on chromatin. Coupling of these two methods (the FLAG tagged TETON inducible sys-tem and RIME) allowed us to control the level of TCF7L1 expression in hESCs, immunopreci-pate TCF7L1 using an anti-FLAG antibody and capture TCF7L1-bound associated complexes on chromatin. Our MS analysis identified some known proteins that have been shown to associate with the WNT/beta-CATENIN/TCF/LEF pathway, as well as novel complexes that have not been linked with TCF7L1. Gene Ontology analysis suggest these proteins function in chromatin modi-fication, splicing, and RNA processing. Our data could create new ideas for in-depth studies of TCF7L1 controlling pluripotency in human ESCs and for understanding how TCF7L1 may act in other cell types.

2024-06-16 | PXD047582 | Pride

RNA-sequencing of LEUTX-inducible H9 human embryonic stem cells

Project description:We generated an inducible DDdCas9VP192 activator cell line in H9 human embryonic stem cells using the Sleeping beauty transposition system. The guides targeting a LEUTX promoter and enhancers were introduced by PiggyBac transposition. Three sample types were generated: Promoter only, Promoter+Enhancer1, Promoter+Enhancer2. Cells were collected before and at 24 h, 48 h, and 72 h after doxycycline treatment, and subjected to STRT RNA-sequencing.

2023-02-12 | E-MTAB-10539 | biostudies-arrayexpress

Genome-wide maps of ARID1A binding genes in H9 human embryonic stem cells

Project description:Genome-wide maps of ARID1A binding genes in H9 human embryonic stem cells

| PRJNA638954 | ENA

Genome-wide maps of ARID1A binding genes in H9 human embryonic stem cells.

Project description:Genome-wide maps of ARID1A binding genes in H9 human embryonic stem cells.

| PRJNA578922 | ENA

Telomeres reforged with non-telomeric sequences in mouse embryonic stem cells (Iso-seq)

Project description:Telomeres reforged with non-telomeric sequences in mouse embryonic stem cells (Iso-seq)

| PRJNA622531 | ENA