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Genomewide screening for pectoral fin MN specific markers

ABSTRACT: Genomewide screening for pectoral fin MN specific markers

PROVIDER: PRJNA414974 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6189921_1.fastq.gz	Fastqsanger.gz
	SRR6189921_2.fastq.gz	Fastqsanger.gz
	SRR6189922_1.fastq.gz	Fastqsanger.gz
	SRR6189922_2.fastq.gz	Fastqsanger.gz
	SRR6189923_1.fastq.gz	Fastqsanger.gz

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Genomewide screening for pectoral fin MN specific markers

Project description:The goal of this study is to find skate pectoral fin motor neuron markers. Total RNA was extracted from manually sorted pectoral fin MNs, which were retrogradely labeled and tail spinal cord cells. By comparing RNA expression profiles of pectoral fin MNs and tail spinal cord neurons, we could identity general MN, MN columnar, and subset MN pool markers for fin MNs.

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Project description:To identify gender specific differences in gene expression during fin rgeneration, pectoral fins were amputated from both male and female adult fish. Fins were allowed to recover for 4 days in standard tank condtions then tissue was collected for RNA isolation and microarray analysis Pooled samples from male and females, injured and uninjured in biological trilicate.

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