ABSTRACT: Metatranscriptome of Poplar tremula x Poplar alba 717-1B4 inoculated with Cenococcum geophilum - Myc Cg1.58_Poplar_latecontact_3R metatranscriptome
Project description:Metatranscriptome of Poplar tremula x Poplar alba 717-1B4 inoculated with Cenococcum geophilum - Myc Cg1.58_Poplar_earlycontact_2R metatranscriptome
Project description:Metatranscriptome of Poplar tremula x Poplar alba 717-1B4 inoculated with Cenococcum geophilum - Myc Cg1.58_Poplar_latecontact_1R metatranscriptome
Project description:Metatranscriptome of Poplar tremula x Poplar alba 717-1B4 inoculated with Cenococcum geophilum - Myc Cg1.58_Poplar_earlycontact_1R metatranscriptome
Project description:Metatranscriptome of Poplar tremula x Poplar alba 717-1B4 inoculated with Cenococcum geophilum - Myc Cg1.58_Poplar_latecontact_2R metatranscriptome
Project description:Metatranscriptome of Cenococcum geophilum from Champenoux, France - Poplar tremula x Poplar alba 717-1B4 mycorrhiza - Myc Cg1.58_Poplar_earlycontact_3Rb metatranscriptome
Project description:The Poplar transcriptome was analyzed in Populus tremulaxPopulus alba clone 717-1B4 control roots and in two poplar lines overexpressing MiSSP7.
Project description:The Poplar transcriptome was analyzed in Populus tremulaxPopulus alba clone 717-1B4 control roots and in two poplar lines overexpressing MiSSP7. We performed 9 hybridizations (NimbleGen) with samples derived from Populus tremulaxPopulus alba clone 717-1B4 control roots, as well as from roots of LINE1 and LINE2 MiSSP7 overexpressor poplars (3 biological replicates each). All samples were labeled with Cy3.
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Populus tremula x alba INRA 717-1B4 roots treated with Methyl jasmonate. Samples were harvested after two weeks either from untreated control roots or from Methyl jasmonate treated roots. Paired-end (2X100bp) reads were generated and aligned to the Populus trichocarpa (http://www.phytozome.net/poplar.php) using CLC Genomics Workbench 6. mRNA profiles from Populus tremula x alba INRA 717-1B4 roots treated with Methyl jasmonate as well as from control roots were generated by paired-end (2X100bp) Illumina HiSeq sequencing. Four samples were sequenced per lane, two biological replicates per treatment.
Project description:Illumina technology was used to generate mRNA profiles of galls from root-knot nematodes infected and corresponding uninfected roots from Poplar CAD and WT lines. RNA was extracted from 3 replicates.TruSeq mRNA Stranded libraries were constructed and after pooling and normalization of libraries, sequencing was done on a NextSeq500 Sequencing System. Raw reads were trimmed for quality and mapped to the substituted genome sequence of P. tremula x P. alba 717-1B4 using CLC Genomics Workbench v9.5.2 and the primary transcripts only.
Project description:The Poplar transcriptome was analyzed in mycorrhizal root tips in contact with Laccaria bicolor for 2 weeks. During mycorrhization the roots were treated with either 250M-BM-5m ACC, 10nM JA or 500M-BM-5M SA and compared to untreated mycorrhiza or control roots without contact to L. bicolor. In addition the poplar mutants 35S::PttACO1 and 35S::Atetr1 were used We performed 27 hybridizations (NimbleGen) with samples derived from Populus tremula x Populus alba clone 717-1B4 control roots, untreated mycorrhiza, SA-treated mycorrhiza, ACC-treated mycorrhiza and JA-treated mycorrhiza (3 biological replicates each) as well as Populus tremula x Populus tremuloides T89 control roots, mycorrhiza, 35S::PttACO1 mycorrhiza and 35S::Atetr1-1 mycorrhiza (3 biological replicates). All samples were labeled with Cy3.
