Project description:Small RNAs are the non-coding RNAs known to regulate various biological functions such as stress adaptation, metabolism, virulence as well as pathogenicity across wide range of bacteria, mainly by controlling mRNA stabilization or regulating translation. Identification and functional characterization of sRNAs that has been carried out in various plant growth promoting bacteria have shown to help the bacterial cell cope up with environmental stress. Till now no study has been carried out to uncover these regulatory molecules in diazotrophic alpha-proteobacterium Azospirillum brasilense Sp245. RNA-Seq is a suitable approach for expression-based sRNA identification in bacteria.
Project description:Canola plants inoculated with plant growth-promoting bacteria either expressing ACC deaminase or not to determine the effect on plant gene expression using an Arabidopsis microarray.
Project description:Canola plants inoculated with plant growth-promoting bacteria either expressing ACC deaminase or not to determine the effect on plant gene expression using an Arabidopsis microarray. 3 replicates for each ACD+ and ACD- bacteria, each compared with untreated control.
