Ontology highlight
ABSTRACT:
PROVIDER: PRJNA422968 | ENA |
REPOSITORIES: ENA
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Project description:Reference genome of Cichorium intybus
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Project description:Transcriptome of Catalogna chicory (Cichorium intybus L. 'Catalogna' group)
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Project description:Differential expression of certain genes during somatic embryogenesis in leaf explants of Cichorium intybus
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Project description:In Cichorium intybus, macroscopic and histological modifications were identified during in vitro cell reactivation and morphogenesis events. The relationships between these modifications and transcript profiles of genes were investigated. Two chicory genotypes derived from the Hungarian landrace Koospol, were tested: K59 is a responsive genotype (embryogenic), as it undergoes cell de- and re-differentiation that leads to somatic embryogenesis (SE). C15 is an unresponsive genotype (nonembryogenic), unable to express this morphogenetic pattern. Previously studies showed that addition of beta-D-glucosyl Yariv reagent (BGlcY), a synthetic phenyl-glycoside that specifically binds arabinogalactan-proteins (AGPs), to the culture medium blocked somatic embryogenesis in chicory root explants in a concentration-dependent manner with complete inhibition of induction occurring at 250 µM of BGlcY (Chapmann et al. 2000a). A putative AGP (DT212818) encoding gene was previously found significantly over-expressed in the K59 as compared to the C15 and was suggested to be involved in chicory re-differentiation. To better understand the cell reactivation events, a treatment with BGlcY was applied in order to precipitate AGPs in muro and to block the in vitro cell re-differentiation processes. The two different genotypes together with BGlcY represented an original tool to discriminate cell reactivation events from cell fate determination. Microscopy, biochemical and transcriptome analyses showed that in vitro SE in chicory was blocked during BGlcY-treatment but cell reactivation still occurred. Consequently, AGP (DT212818) seems to be only important in cell fate determination leading to SE commitment. Gene expression profiles showed in addition to the AGP, other proteins could play a role in SE determination: 26S proteasome AAA ATPase subunit 6 (RPT6), remorin (REM), metallothionein 1 (MT1), 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase). Cell wall events during BGlcY-treatment were observed and discussed.
2009-04-06 | GSE15502 | GEO
Project description:Aster yellows phytoplasma strain Hyd35 (16SrI-B) in micropropagated periwinkle shoots in collection was used to produce infected plants in pots that were separated according to the diverse symptomatology i.e. phyllody and witches’ broom. Small RNA high-throughput sequencing (HTS) was then used to determine the small RNA pattern of these plants. Bioinformatics analysis revealed the presence of expression changes of different miRNA classes and the presence of phytoplasma derived small RNAs. These results could complement previous studies and serve as a starting point for small RNA omics in phytoplasma research
2023-08-11 | GSE213754 | GEO
Project description:Parthenium phyllody phytoplasma strain PR08
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