Project description:Background: Different diets result in significantly different phenotypes through metabolic and genomic reprogramming. Epigenetic marks, identified in humans and mouse models upon caloric restriction, high fat diet or the intake of specific bioactives, suggest that genomic reprogramming drives this reprogramming and mediates the effect of nutrition on health. Histone modifications encode the epigenetic signal that adapts genome functions to environmental conditions, including diets, by tuning the structure and properties of chromatin. So far, the effect of different diets on the genome-wide distribution of critical histone marks has not been determined Methods: We investigated by chromatin immunoprecipitation sequencing the distribution of the trimethylation of lysine 4 of histone H3 in the liver of mice fed for one year with five different diets including: chow containing corn powder as extra source of plant bioactives or specifically enriched with the cyanidin-3-O-glucoside, high fat enriched obesogenic and caloric restricted pro-longevity diets Conclusions: The comparison of the resulting histone mark profiles revealed functional food containing cyanidin determines broad effect.
Project description:Drosophila melanogaster larvae reared on isocaloric diets with different protein/sugar ratios, exhibit different developmental times and the eclosed adults show different metabolite pools of glycogen and triglycerides (Matzkin et al., 2011, PMID: 21525254). To investigate the effect of larval diet on adult neurological processes at the gene expression level we performed high throughout RNA sequencing of fly heads reared in two different protein/sugar ratio diets.
Project description:The effects of different diets on bovine serum extracellular vesicle (EV)-miRNAs are explored by small RNA Solexa sequencing. We partly replaced alfalfa hay with whole cotton seed and soybean hull in the feed formula of treat cows. Small RNAs are enriched in bovine serum EVs, including miRNAs, snRNAs, tiRNAs, Cis-regulatory elements, piRNAs, etc. Totally 359 bos taurus miRNAs are identified by sequencing. There are 15 immune-related miRNAs in the top 20 serum EV-miRNAs, accounting for about 80% of the total. Seven differently expressed known miRNAs were detected in responding to different diets. KEGG analysis showed differently expressed miRNAs are related to hormone signal pathways and protein metabolism.
