Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Paulinella chromatophora

ABSTRACT: A freshwater ameoboid with a cyanobacterium endosymbiont

PROVIDER: PRJNA43261 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR061197_1.fastq.gz	Fastqsanger.gz
	DRR061197_2.fastq.gz	Fastqsanger.gz
	DRR061198_1.fastq.gz	Fastqsanger.gz
	DRR061198_2.fastq.gz	Fastqsanger.gz
	DRR061369.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 15

Similar Datasets

Proteome of an early evolutionary stage photosynthetic organelle in the amoeba Paulinella chromatophora

Project description:The endosymbiotic acquisition of mitochondria and plastids >1 Ga ago profoundly impacted eukaryote evolution. Early stages of organelle integration however remain poorly understood. The amoeba Paulinella chromatophora contains more recently established cyanobacterium-derived photosynthetic organelles, termed “chromatophores”. To explore the re-arrangement of an organellar proteome during its integration into a eukaryotic host cell, we characterized the chromatophore proteome by mass spectrometry. Apparently, genetic control over the chromatophore has shifted substantially to the nucleus. Two classes of nuclear-encoded proteins are imported into the chromatophore, likely through independent pathways. Most imported proteins seem to derive from ancestral host genes rather than nuclear genes transferred from the endosymbiont. Intriguingly, the putative targeting signal found in one class of imported proteins confers chloroplast localization upon heterologous expression in a plant cell suggesting common features in chromatophore and plastid protein import pathways. Finally, combining experimental data with in silico predictions we provide a comprehensive catalogue of chromatophore-localized proteins.

2017-07-13 | PXD006531 | Pride

The Paulinella chromatophore N-terminome

Project description:The cercozoan amoeba Paulinella chromatophora contains photosynthetic organelles - termed chromatophores - that evolved from a cyanobacterium ~100 million years ago, independently from plastids in plants and algae. Despite its more recent origin, at least one third of the chromatophore proteome consists of nucleus-encoded proteins that are imported by an unknown mechanism across the chromatophore double envelope membranes. Chromatophore-targeted proteins fall into two classes. Proteins exceeding 250 amino acids carry a conserved N-terminal sequence extension, termed the ‘chromatophore transit peptide’ (crTP), that is presumably involved in guiding these proteins into the chromatophore. Short imported proteins do not carry discernable targeting signals. To explore whether the import of protein is accompanied by their N-terminal processing, here we used a mass spectrometry-based approach to determine protein N-termini in Paulinella chromatophora and identified N-termini of 208 chromatophore-localized proteins. Our study revealed extensive N-terminal modifications by acetylation and proteolytic processing in both, the nucleus and chromatophore-encoded fraction of the chromatophore proteome. Mature N-termini of 37 crTP-carrying proteins were identified, of which 30 were cleaved in a common processing region. Our results imply that the crTP mediates trafficking through the Golgi, is bipartite and surprisingly only the N-terminal third (‘part 1’) becomes cleaved upon import, whereas the rest (‘part 2’) remains at the mature proteins. In contrast, short imported proteins remain largely unprocessed. Finally, this work sheds light on N-terminal processing of proteins encoded in an evolutionary-early-stage photosynthetic organelle and suggests host-derived post-translationally acting factors involved in dynamic regulation of the chromatophore-encoded chromatophore proteome.

2023-04-06 | PXD028527 | Pride

Metabolome Profiling of Synechococcus elogatus PCC 11802

Project description:Metabolomics Analysis of a novel freshwater cyanobacterium, Synechococcus elongatus PCC 11802 isolated by us from Powai Lake, Mumbai, India. PCC 11802 cells were grown under ambient and 1% CO2 conditions and metabolomics data was collected in three biological replicates and two technical replicates (n=6). The study aims to find metabolomics changes in this cyanobacterium at elevated CO2 levels.

2019-05-07 | ST001180 | MetabolomicsWorkbench

cyanobacterium endosymbiont of Epithemia turgida isolate EtSB Lake Yunoko

Project description:Genome sequencing of cyanobacterium endosymbiont of Epithemia turgida

| PRJDB498 | ENA

Paulinella

Project description:Paulinella overview

| PRJNA186987 | ENA

Paulinella

Project description:Refseqs for Paulinella chromatophora

| PRJNA79543 | ENA

Expression of Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans genes (control vs self-cured)

2015-07-01 | GSE55991 | GEO

Expression of Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans genes

Project description:Transcriptome analysis of Wigglesworthia glossinidia endosymbiont derived from uninfected and infected samples at 3 time points (3, 10 and 20 days). Expression profiling by array - Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans

2015-03-10 | GSE55931 | GEO

Expression of Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans genes (control vs self-cured)

Project description:Transcriptome analysis of Wigglesworthia glossinidia endosymbiont derived from control samples with or without parasite contact at 10 days. Expression profiling by array - Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans RNAs are a mix of Wigglesworthia, Sodalis and glossina. RNAs were extracted from 8 samples including 2 conditions (with 4 replicates per condition).

2015-07-01 | E-GEOD-55991 | biostudies-arrayexpress

Paulinella micropora

Project description:Genome- and transcriptome-analysis of the photosynthetic testate amoeba Paulinella micropora

| PRJDB3528 | ENA