Sarcophaga crassipalpis
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA43267 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Sarcophaga crassipalpis transcriptome sequencing project
| PRJNA48391 | ENA
Project description:Transcriptional profiling of Sarcophaga crassipalpis pupal diapause
| PRJNA125139 | ENA
Project description:Nasonia vitripennis injects venom into its host organism Sarcophaga crassipalpis together with the eggs in order to make it suitable for the offspring to survive. The venom is known to suppress the hosts immune system, elevate the lipid levels, slow down development, et cetera.This microarray can uncover new transcriptomal effects on the host organism after natural envenomation that have not been discovered by bioassays. Since transcriptomal effects will vary during time, two different time points have been selected, 3 and 25 hours after parasitization.
2014-07-16 | GSE36996 | GEO
Project description:Sarcophaga crassipalpis transcriptome project
| PRJNA31359 | ENA
Project description:The overall effect of parasitization by Nasonia vitripennis on its host Sarcophaga crassipalpis
| PRJNA157979 | ENA
Project description:Nasonia vitripennis injects venom into its host organism Sarcophaga crassipalpis together with the eggs in order to make it suitable for the offspring to survive. The venom is known to suppress the hosts immune system, elevate the lipid levels, slow down development, et cetera.This microarray can uncover new transcriptomal effects on the host organism after natural envenomation that have not been discovered by bioassays. Since transcriptomal effects will vary during time, two different time points have been selected, 3 and 25 hours after parasitization. 16 individuals per sample, 4 replicates per group, loopdesign, 4 control individuals per time point, dye swap
2014-07-16 | E-GEOD-36996 | biostudies-arrayexpress
Project description:Small RNA-seq reveals miRNAs that may regulate pupal diapause in the flesh fly, Sarcophaga bullata
| PRJNA369526 | ENA
Project description:The ability to rapidly respond to changes in temperature is critical for insects and other ectotherms living in variable environments. In a physiological process termed rapid cold-hardening (RCH), exposure to non-lethal low temperature allows many insects to significantly increase their cold tolerance in a matter of minutes to hours. Additionally, there are rapid changes in gene expression and cell physiology during recovery from cold injury, and we hypothesize that RCH may modulate some of these processes during recovery. In this study, we used a cDNA microarray to examine the molecular mechanisms of RCH and cold shock (CS) recovery in the flesh fly, Sarcophaga bullata. With our custom 2-color array, we measured expression of ~15,000 ESTs during RCH and during recovery from cold shock. Surprisingly, no transcripts were upregulated during RCH, and likewise, RCH had a minimal effect on the transcript signature during recovery from cold shock. However, during recovery from cold shock, we observed differential expression of ~1,400 ESTs, including a number of heat shock proteins, cytoskeletal components, and genes from several cell signaling pathways. Several gene pathways correlated well with metabolomics data, indicating that coordinated changes in gene expression and metabolism contribute to recovery from cold shock.
2012-03-14 | GSE36483 | GEO
Project description:The ability to rapidly respond to changes in temperature is critical for insects and other ectotherms living in variable environments. In a physiological process termed rapid cold-hardening (RCH), exposure to non-lethal low temperature allows many insects to significantly increase their cold tolerance in a matter of minutes to hours. Additionally, there are rapid changes in gene expression and cell physiology during recovery from cold injury, and we hypothesize that RCH may modulate some of these processes during recovery. In this study, we used a cDNA microarray to examine the molecular mechanisms of RCH and cold shock (CS) recovery in the flesh fly, Sarcophaga bullata. With our custom 2-color array, we measured expression of ~15,000 ESTs during RCH and during recovery from cold shock. Surprisingly, no transcripts were upregulated during RCH, and likewise, RCH had a minimal effect on the transcript signature during recovery from cold shock. However, during recovery from cold shock, we observed differential expression of ~1,400 ESTs, including a number of heat shock proteins, cytoskeletal components, and genes from several cell signaling pathways. Several gene pathways correlated well with metabolomics data, indicating that coordinated changes in gene expression and metabolism contribute to recovery from cold shock. Four treatment groups (C, RCH, CS+2R, RCH+CS+2R), four biological replicates of four pooled individuals for each treatment. Each phenotype was hybridized with the control, and the CS+2R and RCH+CS+2R groups were also hybridized together.
2012-03-14 | E-GEOD-36483 | biostudies-arrayexpress