Long non-coding RNAs associated with metabolic traits in human white adipose tissue
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ABSTRACT:
PROVIDER: PRJNA434431 | ENA |
REPOSITORIES: ENA
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Project description:Long non-coding RNAs (lncRNAs) belong to a recently discovered class of molecules proposed to regulate various cellular processes. Here, we systematically analyzed their expression in human subcutaneous white adipose tissue (WAT) and found that a limited set was differentially expressed in either obesity or the insulin resistance that usually follows obesity. Two lncRNAs herein termed adipocyte-specific metabolic related lncRNAs 1(ASMER-1) and ASMER-2 were enriched in adipocytes and regulated by both obesity and insulin resistance. Knockdown of either ASMER-1or ASMER-2 by antisense oligonucleotides using in vitro differentiated human adipocytes altered lipolysis and adiponectin secretion, respectively. The observed effects could be attributed to changes in the expression of genes controlling inflammation and lipid metabolism. Our data demonstrate that lncRNAs are modulators of the metabolic and endocrine functions in fat cells and also provide an emerging link between WAT and common metabolic conditions.
2021-02-14 | GSE110729 | GEO
Project description:Long non-coding RNAs (lncRNAs) belong to a recently discovered class of molecules proposed to regulate various cellular processes. Here, we systematically analyzed their expression in human subcutaneous white adipose tissue (WAT) and found that a limited set was differentially expressed in obesity and/or the insulin resistant state. Two lncRNAs herein termed adipocyte-specific metabolic related lncRNAs, ASMER-1 and ASMER-2 were enriched in adipocytes and regulated by both obesity and insulin resistance. Knockdown of either ASMER-1 or ASMER-2 by antisense oligonucleotides in in vitro differentiated human adipocytes revealed that both genes regulated adipogenesis, lipid mobilization and adiponectin secretion. The observed effects could be attributed to crosstalk between ASMERs and genes within the master regulatory pathways for adipocyte function including PPARG and INSR. Altogether, our data demonstrate that lncRNAs are modulators of the metabolic and secretory functions in human fat cells and provide an emerging link between WAT and common metabolic conditions.
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Project description:The aim of this study was to identify novel long noncoding RNAs (lncRNAs) that are differentially expressed in the subcutaneous region either in obesity or insulin resistance. A number of novel lncRNAs were differentially expressed in the subcutaneous region either in obesity or insulin resistance. Two lncRNAs (termed here as adipocyte specific insulin resistance related lncRNAs (ADLNRIs), ADLNRI 1 and ADLNRI 2) were influenced by both conditions and enriched in adipocytes. Knockdown of either by antisense oligonucleotides in fat cellsadipocytes altered lipolysis and adiponectin secretion, respectively.
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Project description:White adipose tissue is a central place to energy storage and a major endocrine organ. However, adipose molecular mechanisms have been poorly studied during prolonged fasting. To fill this gap, the aim of this study was to decipher proteomic regulations in rat adipose tissue during phase 2 (lipid mobilization) and phase 3 (protein catabolism) of prolonged fasting compared to the fed state. Specific responses reflecting adipose tissue inflammation, increased fibrinolysis and a possible protein catabolism-related energy saving mechanism were recorded during phase 3. Differences between internal and subcutaneous adipose tissues were essentially related to lipid metabolism, the response to oxidative stress and energy production. These data thus provide a molecular basis of adipose tissue responses according to the fasting stage.
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Project description:The aim of this study was to identify genes expressed selectively in brown adipose tissue as compared to white adipose tissue from the same animals. This analysis provides a gene set that is brown and white adipose selective. Experiment Overall Design: Interscapular brown adipose tissue and epididymal white adipose tissue was carefully dissected from 3 male C57Bl/6 mice. These samples were profiled independently using Affymetrix mouse 430_2 gene arrays, representing 3 biological replicates for each brown and white adipose tissues.
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