Project description:Purpose: The goals of this study are to elucidate the underlying mechanism for the activation of HSCs Methods: After wild type (WT) HSCs (CD150+ CD48- EPCR+ Lineage-) were treated with 5-fluorouracil (5-FU), 100 cells of HSCs were sorted. Moreover, after the culture with or without Nifedipien, cultured HSC fraction (CD150+ CD48- EPCR+ c-kit+ Sca-1+ Lineage-) were sorted. These cells were subjected to mRNA sequence using Next-seq (n>3). The sequence reads that passed quality filters were analyzed by CLC genomic workbench.
Project description:In this study, mRNA expression profiles were examined by Illumina microarray in mouse embryonic stem cells (ESCs) derived from androgenetic (aESC), parthenogenetic (pESC) and fertilized (fESC) blastocysts. Results showed that 2394, 87 and 1788 mRNAs were differentially expressed in the aESCs vs. fESCs, pESCs vs. fESCs and aESCs vs. pESCs, respectively. Androgenetic, parthenogenetic and fertilized embryonic stem cell lines were established from androgenetic, parthenogenetically activated and fertilized blasotocyst. mRNA microarrays were repeated three times using passages 6, 7 and 8.