Expression profile of adult mouse POA/BF regions
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ABSTRACT:
PROVIDER: PRJNA437139 | ENA |
REPOSITORIES: ENA
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Project description:The adult mammalian brain is composed of distinct regions that have specialized roles. The BF/POA regions are thought to have an important role in the regulation of sleep/wake behavior. However, genetic markers of the responsible cells for the regulation of sleep/wake behavior are largely unknown. To identify the molecular markers of the BF/POA regions, we sampled the BF/POA regions and compared gene expression in the BF/POA regions with those of other brain regions which we previously reported in the BrainStars (B*) project, in which we sampled ~50 small brain regions, including sensory centers and centers for motion, time, memory, fear, and feeding. We sampled each region every 4 hours for 24 hours, and pooled the sample sets for DNA-microarray assays. We then used informatics to identify candidates for genes with high or low expression in the BF/POA regions. We used our findings to develop an integrated database (http://poabf.brainstars.org/) for exploring genome-wide expression in the adult mouse brain including the BF/POA regions.
2018-08-17 | GSE107500 | GEO
Project description:The proliferative niches in the subpallium generate a rich cellular variety fated for diverse telencephalic regions. The embryonic preoptic area (POA) represents one of these domains giving rise to the pool of cortical GABAergic interneurons and glial cells, in addition to striatal and residual POA cells. The migration from sites of origin within the subpallium to the distant targets like the cerebral cortex, accomplished by the adoption and maintenance of a particular migratory morphology, is a critical step during interneuron development, which seems to be regulated partially via DNA methylation-dependent gene expression. To identify genes that are altered by DNA methylation mediated by DNMT1 in POA-derived Hmx3-positive interneurons, we used an Hmx3-Cre/tdTomato/Dnmt1loxP mouse model and FAC-sorted the basal telencephalon at E16. RNA and MeDIP sequencing were performed. MeDIP data are assigned here.
2021-03-09 | GSE146967 | GEO
Project description:The proliferative niches in the subpallium generate a rich cellular variety fated for diverse telencephalic regions. The embryonic preoptic area (POA) represents one of these domains giving rise to the pool of cortical GABAergic interneurons and glial cells, in addition to striatal and residual POA cells. The migration from sites of origin within the subpallium to the distant targets like the cerebral cortex, accomplished by the adoption and maintenance of a particular migratory morphology, is a critical step during interneuron development, which seems to be regulated partially via DNA methylation-dependent gene expression. To identify genes that are altered by DNA methylation mediated by DNMT1 in POA-derived Hmx3-positive interneurons, we used an Hmx3-Cre/tdTomato/Dnmt1loxP mouse model and FAC-sorted the basal telencephalon at E16. RNA and MeDIP sequencing were performed. RNA data are assigned here.
2021-03-09 | GSE146966 | GEO
Project description:Expression profile of lncRNAs and mRNAs in 4 brain regions of adult mouse
| PRJNA418153 | ENA
Project description:We have shown that pre-incubation with Bifidobacterium bifidum (B. bifidum) strain BF-1, a probiotic strain known to improve H. pylori-associated gastritis, suppresses the induction of IL-8 by the pathogen. To investigate how BF-1 affects gene expression in H. pylori-infected cells, we performed microarray analysis to assess gene expression in epithelial cells, which had been pre-incubated with BF-1 and infected with H. pylori.
2010-10-13 | GSE20171 | GEO
Project description:The adult mammalian brain is composed of distinct regions that have specialized roles. To dissect molecularly this complex structure, we conducted a project, named the BrainStars (B*) project, in which we sampled ~50 small brain regions, including sensory centers and centers for motion, time, memory, fear, and feeding. To avoid confusion from temporal differences in gene expression, we sampled each region every 4 hours for 24 hours, and pooled the sample sets for DNA-microarray assays. Therefore, we focused only on spatial differences in gene expression. We then used informatics to identify candidates for (1) genes with high or low expression in specific regions, (2) switch-like genes with bimodal or multimodal expression patterns, and (3) genes with a uni-modal expression pattern that exhibit stable or variable levels of expression across brain regions. We used our findings to develop an integrated database (http://brainstars.org/) for exploring genome-wide expression in the adult mouse brain.
2011-08-12 | GSE16496 | GEO