Project description:We report the application of single-molecule-based sequencing technology for high-throughput profiling of histone modifications in mycobacterium smegmatis C2 155. By obtaining over four billion bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of Mycobacterium smegmatis C2 155. We find that amino acids synthesis and degradation genes, genes that are expressed.The ratio of gene expression based on transcriptome substantiate an enhanced TCA cycle. The upregulation of Lys1 was found to prompt this degradation of lysine. The transformation from glutamine to glutamate as well as arginine was mediated by glsA (MSMEG_3818) , of which glutamate engaged into the TCA cycle via α-ketoglutarate. The phenylalanine was degraded into fumarate via mhpB. The succinate formation from asparate degradation was mediated by methylcitrate dehydratase (MSMEG_6645), carboxyvinyl-carboxyphosphonate phosphorylmutase (MSMEG_2506) and MSMEG_6855. and therefore reflect metabolism state of strains. Data from sample treated with rifampicin/glutamine reveals the upregulation of respiration.
Project description:Interventions: capecitabine
Primary outcome(s): The correlation between the capecitabine dose and the C1 and C2 levels of 5-FU, unchanged capecitabine, 5’-DFCR, 5’-DFUR in the blood. C1=concentration an hour after administration of capecitabine C2=concentration two hours after administration of capecitabine.
Study Design: Single arm Non-randomized
Project description:affy_meja_arabidopsis - We want to determine if the presence of C2 modifies the cell transcriptome and its response to methyl jasmonate. Comparison of control lines and transgenic lines expressing C2 in basal conditions and after treatment with 50uM MeJA for 10 hours. C2 lines are expressing geminivirus C2 under a 35S promoter. PR1-LUC have a luciferase transgene under the control of the promoter of PR1; since the plants are not induced, these are the control plants (resistant to kanamycin, but not expressing anything else) Keywords: gene knock in (transgenic),treated vs untreated comparison
Project description:The aim of the study was to determine biological relevance of differentially expressed genes in Lactobacillus plantarum C2 during fermentation of plant substrates. Whole-transcriptome analysis based on customized microarray profiles has been used to determine altered transcription patterns in L. plantarum C2.
Project description:Cotyledons and leaf transcriptomes of species of Salsoleae with different photosynthetic types were de novo assembled and analyzed to provide a better understanding of differential gene expression between C3, C2 and C4 species. Total RNA of cotyledons and leaves of different species of Salsoleae with different photosynthetic types (C3 Salsola webbii, C2 Salsola divaricata, C4 Salsola oppositifolia, C4 Hammada scoparia) were isolated with RNeasy Plant Mini Kit (Qiagen) following Standard protocol (January 2011) and including DNase Digestion with RNase-Free DNase Set (Qiagen). 500 ng were used for cDNA library generation conducted with TruSeq RNA Sample Preperation Kit (Illumina Inc.) following Low Sample Protocol (TruSeq RNA Sample Preparation v2 Guide, Illumina Proprietary, Part # 15026495 Rev. C, May 2012). Sequencing of single reads was performed on an Illumina HiSeq2000 platform.
